Journal article
Protocol to extract tear fluid for proteomics using Schirmer strips
STAR protocols, Vol.6(4), 104146
12/19/2025
DOI: 10.1016/j.xpro.2025.104146
PMCID: PMC12554168
PMID: 41108683
Abstract
Schirmer strips are widely regarded as the gold standard for tear fluid collection. However, their use presents several challenges for proteomic analysis. Here, we present a protocol for extracting tear proteins from Schirmer strips. We describe steps for acquisition and handling of strips, extraction buffer preparation, strip preparation, and protein extraction. This protocol is designed to improve protein yield and facilitate proteomic workflows and is adaptable for various protein-based studies, particularly in the context of ocular disease research and diagnostics.
Details
- Title: Subtitle
- Protocol to extract tear fluid for proteomics using Schirmer strips
- Creators
- Gia-Han Ngo - Palo Alto UniversityMadhumeeta Chadha - Stanford UniversityYoung Joo Sun - Stanford UniversityGina Yu - Stanford UniversitySoo Hyeon LeeTsai-Chu Yeh - Palo Alto UniversityDavid R P Almeida - The CentersAlexander G Bassuk - University of IowaPrithvi Mruthyunjaya - Stanford UniversityAntoine Dufour - University of CalgaryVinit B Mahajan - Stanford University
- Resource Type
- Journal article
- Publication Details
- STAR protocols, Vol.6(4), 104146
- DOI
- 10.1016/j.xpro.2025.104146
- PMID
- 41108683
- PMCID
- PMC12554168
- NLM abbreviation
- STAR Protoc
- ISSN
- 2666-1667
- eISSN
- 2666-1667
- Publisher
- Elsevier
- Grant note
- NIH: R01EY031952, R01EY030151, R01EY024665, R01EY025225, P30EY026877 Stanford ChEM-H IMAStanford Center for Optic Disc DrusenResearch to Prevent Blindness, United StatesSTARS award by the Arthritis Society of CanadaCanadian Institutes of Health Research (CIHR, Canada): 449589 Natural Science and Engineering Council of Canada (NSERC, Canada): DGECR-2019-00112 Alan and Irene Adler Ocular Research InitiativeMills and Margaret Cox Macula Society Research/Retina Research Foundation, United StatesBright Focus Foundation's Macular Degeneration Research program
V.B.M. is supported by NIH grants (R01EY031952, R01EY030151, R01EY024665, R01EY025225, and P30EY026877) , Stanford ChEM-H IMA, the Stanford Center for Optic Disc Drusen, and Research to Prevent Blindness, United States. A.D. is supported by the STARS award by the Arthritis Society of Canada, the Canadian Institutes of Health Research (CIHR, Canada) (grant no. 449589) , and the Natural Science and Engineering Council of Canada (NSERC, Canada) (grant no. DGECR-2019-00112) . V.B.M. and P.M. are supported by the Alan and Irene Adler Ocular Research Initiative and the Mills and Margaret Cox Macula Society Research/Retina Research Foundation, United States. Y.J.S. is supported by Bright Focus Foundation's Macular Degeneration Research program. We thank the Seer Inc. team for processing the tear samples. Biorender soft ware was used to create the figure under an academic license
- Language
- English
- Electronic publication date
- 10/16/2025
- Date published
- 12/19/2025
- Academic Unit
- Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
- Record Identifier
- 9985016011402771
Metrics
29 Record Views