Journal article
Protocols for Generating Surfaces and Measuring 3D Organelle Morphology Using Amira
Cells (Basel, Switzerland), Vol.11(1), 65
12/27/2021
DOI: 10.3390/cells11010065
PMCID: PMC8750564
PMID: 35011629
Abstract
High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.
Details
- Title: Subtitle
- Protocols for Generating Surfaces and Measuring 3D Organelle Morphology Using Amira
- Creators
- Edgar Garza Lopez - Hinton and Garza Lopez Family Consulting Company, Iowa City, IA 52246, USAZer Vue - Vanderbilt UniversityPrasanna Katti - National Heart Lung and Blood InstituteKit Neikirk - University of Hawaii at HiloMichelle Biete - University of Hawaii at HiloJacob Lam - University of IowaHeather K. Beasley - Vanderbilt UniversityAndrea G. Marshall - Vanderbilt UniversityTaylor A. Rodman - Vanderbilt UniversityTrace A. Christensen - Mayo ClinicJeffrey L. Salisbury - Mayo ClinicLarry Vang - Vanderbilt UniversityMargaret Mungai - University of IowaSalma AshShareef - University of IowaSandra A. Murray - University of PittsburghJianqiang Shao - University of IowaJennifer Streeter - University of IowaBrian Glancy - National Heart Lung and Blood InstituteRenata O. Pereira - Univ Iowa, Carver Coll Med, Dept Internal Med, Iowa City, IA 52242 USAE. Dale Abel - University of IowaAntentor Hinton - Vanderbilt University
- Resource Type
- Journal article
- Publication Details
- Cells (Basel, Switzerland), Vol.11(1), 65
- DOI
- 10.3390/cells11010065
- PMID
- 35011629
- PMCID
- PMC8750564
- NLM abbreviation
- Cells
- ISSN
- 2073-4409
- eISSN
- 2073-4409
- Publisher
- Mdpi
- Number of pages
- 16
- Grant note
- name: UNCF/BMS EE Just Investigator Award, award: N/A, N/A
- Language
- English
- Date published
- 12/27/2021
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984420935402771
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