Journal article
Pseudohypericin is necessary for the light-activated inhibition of prostaglandin E2 pathways by a 4 component system mimicking an Hypericum perforatum fraction
Phytochemistry (Oxford), Vol.69(12), pp.2354-2362
2008
DOI: 10.1016/j.phytochem.2008.06.010
PMCID: PMC2633243
PMID: 18707743
Abstract
An anti-inflammatory guided fractionation of Hypericum perforatum (Hp) was used because most Hp research has traditionally focused on its anti-depressant and cytotoxic properties. The combination of 4 constituents (chlorogenic acid, amentoflavone, quercetin, pseudohypericin) explained the activity of a subfraction in light-activated but not dark conditions, and pseudohypericin was necessary but not sufficient for the anti-inflammatory activity. Hypericum perforatum (Hp) has been used medicinally to treat a variety of conditions including mild-to-moderate depression. Recently, several anti-inflammatory activities of Hp have been reported. An ethanol extract of Hp was fractionated with the guidance of an anti-inflammatory bioassay (lipopolysaccharide (LPS)-induced prostaglandin E 2 production (PGE 2)), and four constituents were identified. When combined together at concentrations detected in the Hp fraction to make a 4 component system, these constituents (0.1 μM chlorogenic acid (compound 1), 0.08 μM amentoflavone (compound 2), 0.07 μM quercetin (compound 3), and 0.03 μM pseudohypericin (compound 4)) explained the majority of the activity of the fraction when activated by light, but only partially explained the activity of this Hp fraction in dark conditions. One of the constituents, light-activated pseudohypericin, was necessary, but not sufficient to explain the reduction in LPS-induced PGE 2 of the 4 component system. The Hp fraction and the 4 component system inhibited lipoxygenase and cytosolic phospholipase A 2, two enzymes in the PGE 2-mediated inflammatory response. The 4 component system inhibited the production of the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α), and the Hp fraction inhibited the anti-inflammatory cytokine interleukin-10 (IL-10). Thus, the Hp fraction and selected constituents from this fraction showed evidence of blocking pro-inflammatory mediators but not enhancing inflammation-suppressing mediators.
Details
- Title: Subtitle
- Pseudohypericin is necessary for the light-activated inhibition of prostaglandin E2 pathways by a 4 component system mimicking an Hypericum perforatum fraction
- Creators
- Kimberly D.P Hammer - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesMatthew L Hillwig - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesJeffrey D Neighbors - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesYoung-Je Sim - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesMarian L Kohut - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesDavid F Wiemer - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesEve S Wurtele - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United StatesDiane F Birt - The Center for Research on Botanical Dietary Supplements, 215 MacKay Hall, Ames, IA 50011, United States
- Resource Type
- Journal article
- Publication Details
- Phytochemistry (Oxford), Vol.69(12), pp.2354-2362
- DOI
- 10.1016/j.phytochem.2008.06.010
- PMID
- 18707743
- PMCID
- PMC2633243
- NLM abbreviation
- Phytochemistry
- ISSN
- 0031-9422
- eISSN
- 1873-3700
- Publisher
- Elsevier BV
- Language
- English
- Date published
- 2008
- Academic Unit
- Neuroscience and Pharmacology; Chemistry
- Record Identifier
- 9983985914602771
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