Journal article
Pseudomonas aeruginosa Magnesium Transporter MgtE Inhibits Type III Secretion System Gene Expression by Stimulating rsmYZ Transcription
Journal of bacteriology, Vol.199(23), e00268-17
12/01/2017
DOI: 10.1128/JB.00268-17
PMCID: PMC5686585
PMID: 28847924
Abstract
causes numerous acute and chronic opportunistic infections in humans. One of its most formidable weapons is a type III secretion system (T3SS), which injects powerful toxins directly into host cells. The toxins lead to cell dysfunction and, ultimately, cell death. Identification of regulatory pathways that control T3SS gene expression may lead to the discovery of novel therapeutics to treat
infections. In a previous study, we found that expression of the magnesium transporter gene
inhibits T3SS gene transcription. MgtE-dependent inhibition appeared to interfere with the synthesis or function of the master T3SS transcriptional activator ExsA, although the exact mechanism was unclear. We now demonstrate that
expression acts through the GacAS two-component system to activate
and
transcription. This event ultimately leads to inhibition of
translation. This inhibitory effect is specific to
as translation of other genes in the
operon is not inhibited by
Moreover, our data reveal that MgtE acts solely through this pathway to regulate T3SS gene transcription. Our study reveals an important mechanism that may allow
to fine-tune T3SS activity in response to certain environmental stimuli.
The type III secretion system (T3SS) is a critical virulence factor utilized by numerous Gram-negative bacteria, including
, to intoxicate and kill host cells. Elucidating T3SS regulatory mechanisms may uncover targets for novel anti-
therapeutics and provide deeper understanding of bacterial pathogenesis. We previously found that the magnesium transporter MgtE inhibits T3SS gene transcription in
In this study, we describe the mechanism of MgtE-dependent inhibition of the T3SS. Our report also illustrates how MgtE might respond to environmental cues, such as magnesium levels, to fine-tune T3SS gene expression.
Details
- Title: Subtitle
- Pseudomonas aeruginosa Magnesium Transporter MgtE Inhibits Type III Secretion System Gene Expression by Stimulating rsmYZ Transcription
- Creators
- Shubham Chakravarty - Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana, USACameron N Melton - Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana, USAAdam Bailin - Department of Microbiology, University of Iowa, Iowa City, Iowa, USATimothy L Yahr - Department of Microbiology, University of Iowa, Iowa City, Iowa, USAGregory G Anderson - Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana, USA ga2@iupui.edu
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.199(23), e00268-17
- Publisher
- United States
- DOI
- 10.1128/JB.00268-17
- PMID
- 28847924
- PMCID
- PMC5686585
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Grant note
- R56 AI055042 / NIAID NIH HHS R01 AI055042 / NIAID NIH HHS R01 AI097264 / NIAID NIH HHS P30 DK054759 / NIDDK NIH HHS
- Language
- English
- Date published
- 12/01/2017
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001128902771
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