Journal article
Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane
The Journal of cell biology, Vol.177(1), pp.21-27
04/09/2007
DOI: 10.1083/jcb.200605142
PMCID: PMC2064102
PMID: 17403925
Abstract
Pseudomonas aeruginosa, an important human pathogen, preferentially binds and enters injured cells from the basolateral (BL) surface. We previously demonstrated that activation of phosphatidylinositol 3-kinase (PI3K) and Akt are necessary and sufficient for P. aeruginosa entry from the apical (AP) surface and that AP addition of phosphatidylinositol 3,4,5-trisphosphate (PIP3) is sufficient to convert AP into BL membrane (Kierbel, A., A. Gassama-Diagne, K. Mostov, and J.N. Engel. 2005. Mol. Biol. Cell. 16:2577–2585; Gassama-Diagne, A., W. Yu, M. ter Beest, F. Martin-Belmonte, A. Kierbel, J. Engel, and K. Mostov. 2006. Nat. Cell Biol. 8:963–970). We now show that P. aeruginosa subverts this pathway to gain entry from the AP surface. In polarized monolayers, P. aeruginosa binds near cell–cell junctions without compromising them where it activates and recruits PI3K to the AP surface. Membrane protrusions enriched for PIP3 and actin accumulate at the AP surface at the site of bacterial binding. These protrusions lack AP membrane markers and are comprised of BL membrane constituents, which are trafficked there by transcytosis. The end result is that this bacterium transforms AP into BL membrane, creating a local microenvironment that facilitates its colonization and entry into the mucosal barrier.
Details
- Title: Subtitle
- Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane
- Creators
- Arlinet Kierbel - Department of MedicineAma Gassama-Diagne - Department of Anatomy, Department of Biochemistry and Biophysics,, Institut Federatif de Recherche Claude de Preval, Institut Federatif de Recherche 30, Institut National de la Santé et de la Recherche Medicale Unite 563, Departement Lipoproteines et Mediateurs Lipidiques, Hospital Purpan, 31059 Toulouse, Cedex 3, FranceClaudia Rocha - Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425Lilliana Radoshevich - Department of Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143Joan Olson - Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425Keith Mostov - Department of Anatomy, Department of Biochemistry and BiophysicsJoanne Engel - Department of Medicine, Department of Microbiology and Immunology
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.177(1), pp.21-27
- DOI
- 10.1083/jcb.200605142
- PMID
- 17403925
- PMCID
- PMC2064102
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 04/09/2007
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984025336202771
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