Journal article
Pu-erh black tea supplementation decreases quinocetone-induced ROS generation and oxidative DNA damage in Balb/c mice
Food and chemical toxicology, Vol.49(2), pp.477-484
02/2011
DOI: 10.1016/j.fct.2010.11.028
PMID: 21112366
Abstract
Quinocetone (3-methyl-2-quinoxalinbenzenevinylketo-1,4-dioxide, QCT), a new feed antibacterial agent of quinoxaline-1,4-dioxides family, has been used as an animal growth promoter. However, few data about its potential toxicity in vivo were available. In this study, genotoxicity of QCT and the relationship with oxidative stress were investigated. Balb/c mice with both sexes were administrated with QCT (12000, 6000 and 3000mg/kg/bw, respectively) by gavage acutely. DNA damage, generation of reactive oxygen species (ROS) and activity of antioxidative system (total antioxidative capacity, glutathione, glutathione peroxidase, superoxide dismutase and catalase) in liver and kidney were determined. Moreover, Pu-erh black tea extract (BTE) was co-administrated with QCT to evaluate its protective effect against QCT-induced genotoxicity. The DNA damage was observed in all the groups treated with single QCT except the liver with dose of 3000mg/kg/bw. ROS was accumulated and antioxidative system was suppressed both in liver and kidney. However, the DNA damage, as well as the ROS, was decreased, while the activity of antioxidative system was increased in mice after co-administration of QCT and BTE. These data demonstrate that oxidative stress mediated the genotoxicity induced by QCT in vivo. Furthermore, this oxidative DNA damage can be attenuated by pre-supplementation of BTE.
Details
- Title: Subtitle
- Pu-erh black tea supplementation decreases quinocetone-induced ROS generation and oxidative DNA damage in Balb/c mice
- Creators
- Di Wang - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaYing Zhong - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaXiao Luo - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaShuang Wu - Huazhong University of Science and TechnologyRong Xiao - College of Food Science, Yunnan Agriculture University, Kunming, Yunnan 650201, PR ChinaWei Bao - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaWei Yang - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaHong Yan - MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaPing Yao - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR ChinaLiegang Liu - Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, PR China
- Resource Type
- Journal article
- Publication Details
- Food and chemical toxicology, Vol.49(2), pp.477-484
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.fct.2010.11.028
- PMID
- 21112366
- ISSN
- 0278-6915
- eISSN
- 1873-6351
- Grant note
- DOI: 10.13039/501100012166, name: National Basic Research Program of China, award: 2009CB118803
- Language
- English
- Date published
- 02/2011
- Academic Unit
- Epidemiology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9983995013202771
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