Pulmonary immune responses to Propionibacterium acnes in C57BL/6 and BALB/c mice

Joshua G McCaskill; Kelly D Chason; Xiaoyang Hua; Isabel P Neuringer; Andrew J Ghio; William K Funkhouser; Stephen L Tilley

doi:10.1165/rcmb.2005-0285OC

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Pulmonary immune responses to Propionibacterium acnes in C57BL/6 and BALB/c mice

Journal article

Open access

Peer reviewed

Pulmonary immune responses to Propionibacterium acnes in C57BL/6 and BALB/c mice

Joshua G McCaskill, Kelly D Chason, Xiaoyang Hua, Isabel P Neuringer, Andrew J Ghio, William K Funkhouser and Stephen L Tilley

American journal of respiratory cell and molecular biology, Vol.35(3), pp.347-356

09/2006

DOI: 10.1165/rcmb.2005-0285OC

PMCID: PMC2643288

PMID: 16645181

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Abstract

Propionibacterium acnes (PA) is a gram-positive anaerobic bacterium implicated as a putative etiologic agent of sarcoidosis. To characterize the pulmonary immune response to PA, C57BL/6 and BALB/c mice were intraperitoneally sensitized and intratracheally challenged with heat-killed bacteria. C57BL/6 mice challenged with PA developed a cellular immune response characterized by elevations in Th1 cytokines/chemokines, increased numbers of lymphocytes and macrophages in lung lavage fluid, and peribronchovascular granulomatous inflammation composed of T- and B-lymphocytes and epithelioid histiocytes. T-lymphocytes in the lung lavage fluid showed a marked CD4+ cell predominance. In contrast, C57BL/6 mice challenged with Staphylococcus epidermidis (SE), another gram-positive commensal of human skin, and BALB/c mice challenged with PA, showed only a modest induction of Th1 cytokines, less pulmonary inflammation, and no granulomatous changes in the lung. Enhancement of Toll-like receptor expression was seen in PA-exposed C57BL/6 mice within 24 h after exposure, suggesting that induction of innate immunity by PA contributes to the robust, polarized Th1 immune response elicited by this bacterium. These findings suggest that PA-induced pulmonary inflammation may be a useful model for testing the contributions of both bacterial and host factors in the development, maintenance, and resolution of granulomatous inflammation in the lung.

Animals

Bronchoalveolar Lavage Fluid - cytology

Chemokines - metabolism

Cytokines - metabolism

Gram-Positive Bacterial Infections - immunology

Gram-Positive Bacterial Infections - pathology

Lung - immunology

Lung - microbiology

Lung - pathology

Lymphocytes - immunology

Macrophages - immunology

Mice

Mice, Inbred BALB C

Mice, Inbred C57BL

Peptidyl-Dipeptidase A - metabolism

Pneumonia, Bacterial - immunology

Pneumonia, Bacterial - pathology

Propionibacterium acnes

Th1 Cells - immunology

Toll-Like Receptors - genetics

Toll-Like Receptors - metabolism

Details

Title: Subtitle: Pulmonary immune responses to Propionibacterium acnes in C57BL/6 and BALB/c mice
Creators: Joshua G McCaskill - University of North Carolina at Chapel Hill
Kelly D Chason
Xiaoyang Hua
Isabel P Neuringer
Andrew J Ghio
William K Funkhouser
Stephen L Tilley - Pulmonary
Resource Type: Journal article
Publication Details: American journal of respiratory cell and molecular biology, Vol.35(3), pp.347-356
DOI: 10.1165/rcmb.2005-0285OC
PMID: 16645181
PMCID: PMC2643288
ISSN: 1044-1549
eISSN: 1535-4989
Grant note: HL077581 / NHLBI NIH HHS
Language: English
Date published: 09/2006
Academic Unit: Otolaryngology
Record Identifier: 9984311445702771

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