Journal article
Pulsatile release of biomolecules from polydimethylsiloxane (PDMS) chips with hydrolytically degradable seals
Journal of controlled release, Vol.127(3), pp.280-287
2008
DOI: 10.1016/j.jconrel.2008.02.001
PMID: 18342975
Abstract
We demonstrate, for the first time, a robust novel polydimethylsiloxane (PDMS) chip that can provide controlled pulsatile release of DNA based molecules, proteins and oligonucleotides without external stimuli or triggers. The PDMS chip with arrays of wells was constructed by replica molding. Poly(lactic acid-co-glycolic acid) (PLGA) polymer films of varying composition and thickness were used as seals to the wells. The composition, molecular weight and thickness of the PLGA films were all parameters used to control the degradation rate of the seals and therefore the release profiles. Degradation of the films followed the PLGA composition order of 50:50 PLGA
>
75:25 PLGA
>
85:15 PLGA at all time-points beyond week 1. Scanning electron microscopy images showed that films were initially smooth, became porous and ruptured as the osmotic pressure pushed the degrading PLGA film outwards. Pulsatile release of DNA was controlled by the composition and thickness of the PLGA used to seal the well. Transfection experiments in a model Human Embryonic Kidney 293 (HEK293) cell line showed that plasmid DNA loaded in the wells was functional after pulsatile release in comparison to control plasmid DNA at all time-points. Thicker films degraded faster than thinner films and could be used to fine-tune the release of DNA over day length periods. Finally the PDMS chip was shown to provide repeated sequential release of CpG oligonucleotides and a model antigen, Ovalbumin (OVA), indicating significant potential for this device for vaccinations or applications that require defined complex release patterns of a variety of chemicals, drugs and biomolecules.
Details
- Title: Subtitle
- Pulsatile release of biomolecules from polydimethylsiloxane (PDMS) chips with hydrolytically degradable seals
- Creators
- Janjira IntraJustin M GlasgowHoang Q MaiAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Journal of controlled release, Vol.127(3), pp.280-287
- DOI
- 10.1016/j.jconrel.2008.02.001
- PMID
- 18342975
- NLM abbreviation
- J Control Release
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983986464602771
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