Pulsed Electric Field for protein release of the microalgae Chlorella vulgaris and Neochloris oleoabundans

G. P. T Lam; P. R. Postma; D. A. Fernandes; Renske M. A. Timmermans; Marian H. Vermuë; Maria J. Barbosa; Michel H. M. Eppink; Rene Hubertus Wijffels; Giuseppe Olivieri

doi:10.1016/j.algal.2017.03.024

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Pulsed Electric Field for protein release of the microalgae Chlorella vulgaris and Neochloris oleoabundans

Journal article

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Pulsed Electric Field for protein release of the microalgae Chlorella vulgaris and Neochloris oleoabundans

G. P. T Lam, P. R. Postma, D. A. Fernandes, Renske M. A. Timmermans, Marian H. Vermuë, Maria J. Barbosa, Michel H. M. Eppink, Rene Hubertus Wijffels and Giuseppe Olivieri

Algal Research, Vol.24(Part A), pp.181-187

06/01/2017

DOI: 10.1016/j.algal.2017.03.024

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Abstract

Pulsed Electric Field (PEF) is currently discussed as promising technology for mild and scalable cell disintegration of microalgae. In this study Chlorella vulgaris and Neochloris oleoabundans have been subjected to batch and continuous PEF treatments under a wide range of operating conditions (1–40 pulses, 0.05–5 ms pulses, 7.5–30 kV cm− 1, 0.05–150 kWh kgDW − 1). In many cases after treatment, both algal species show release of ions, which indicates that PEF treatment resulted in permeabilization of the algal cell. However, the electroporation effect was not sufficient to substantially release intracellular proteins. Even at severe energy input (10 to 100 times higher than bead milling) only up to 13% of proteins released from the cells in comparison to 45–50% after bead milling.

Basale biofag

Bead milling

Biorefinery

Cell disintegration

Cellebiologi

Marinbiologi

Matematikk og Naturvitenskap

Microalgae

Pulsed electric field (PEF)

Zoologiske og botaniske fag

Details

Title: Subtitle: Pulsed Electric Field for protein release of the microalgae Chlorella vulgaris and Neochloris oleoabundans
Creators: G. P. T Lam
P. R. Postma - Wageningen University & Research
D. A. Fernandes - Wageningen University & Research
Renske M. A. Timmermans
Marian H. Vermuë - Wageningen University & Research
Maria J. Barbosa
Michel H. M. Eppink - Wageningen University & Research
Rene Hubertus Wijffels - Wageningen University & Research
Giuseppe Olivieri - Wageningen University & Research
Resource Type: Journal article
Publication Details: Algal Research, Vol.24(Part A), pp.181-187
DOI: 10.1016/j.algal.2017.03.024
ISSN: 2211-9264
eISSN: 2211-9264
Publisher: Elsevier
Grant note: name: IPOP Biorefinery; DOI: 10.13039/501100004725, name: Ministry of Economic Affairs
Language: English
Date published: 06/01/2017
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984398019902771

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