Journal article
Purification and Specificity of β1,2-Xylosyltransferase, an Enzyme That Contributes to the Allergenicity of Some Plant Proteins
The Journal of biological chemistry, Vol.272(50), pp.31340-31347
12/12/1997
DOI: 10.1074/jbc.272.50.31340
Abstract
The enzyme that transfersd-xylose from UDP-xylose to the β-linked mannose of plant N-linked oligosaccharides was purified about 51,000-fold to apparent homogeneity from soybean microsomes. On SDS gels, two proteins of 56 and 59 kDa were detected and both were labeled to the same extent by the photoaffinity label, 5-N3-UDP-[32P]xylose. Labeling of both proteins was inhibited by cold UDP-xylose, but not by UDP-glucose. The amount of 5-N3-UDP-[32P]xylose that bound to the two protein bands was greatly increased in the presence of oligosaccharide acceptors. The best acceptor for xylose transfer and for stimulation of UDP-xylose binding was GlcNAc2Man3GlcNAc2-T, but GlcNAc1Man3GlcNAc2, with the GlcNAc on the 3-branch, was also a good acceptor and a good stimulator. A number of other N-linked oligosaccharides were poor acceptors, especially those with galactose units at the nonreducing termini. Many of the properties of this enzyme have been described, and the product of the reaction of UDP-xylose and GlcNAc2Man3(GlcNAc)2 was characterized as GlcNAcβ1, 2Manα1,6(GlcNAcβ1,2Manα1,3)(Xylβ1,2)Manβ1,4GlcNA c2-T by chemical and NMR methods.
Details
- Title: Subtitle
- Purification and Specificity of β1,2-Xylosyltransferase, an Enzyme That Contributes to the Allergenicity of Some Plant Proteins
- Creators
- Yucheng Zeng - University of Arkansas for Medical SciencesGary Bannon - University of Arkansas for Medical SciencesV. Hayden Thomas - University of MichiganKevin Rice - University of MichiganRichard Drake - University of Arkansas for Medical SciencesAlan Elbein - University of Arkansas for Medical Sciences
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.272(50), pp.31340-31347
- DOI
- 10.1074/jbc.272.50.31340
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 12/12/1997
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984365900202771
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