Purification, immunochemical characterization, and immunohistochemical localization of rat hepatic aryl sulfotransferase IV

Michael W Duffel; Thomas P Binder; Lynn Hosie; Holly A Baden; Jill A Sanders; Shirley A Knapp; Jeffrey Baron

doi:10.1016/S0026-895X(25)12849-6

Back

Purification, immunochemical characterization, and immunohistochemical localization of rat hepatic aryl sulfotransferase IV

Journal article

Peer reviewed

Purification, immunochemical characterization, and immunohistochemical localization of rat hepatic aryl sulfotransferase IV

Michael W Duffel, Thomas P Binder, Lynn Hosie, Holly A Baden, Jill A Sanders, Shirley A Knapp and Jeffrey Baron

Molecular pharmacology, Vol.40(1), pp.36-44

1991

DOI: 10.1016/S0026-895X(25)12849-6

PMID: 1857339

View Online

Abstract

Aryl sulfotransferases catalyze the formation of sulfuric acid esters from a diverse group of endogenous and xenobiotic organic chemicals. The isoenzyme of aryl sulfotransferase in livers of male Sprague-Dawley rats that exhibits the most varied substrate specificity is aryl sulfotransferase IV. A new method for the purification to homogeneity of aryl sulfotransferase IV was developed that, when compared with previously described procedures, provided a greater than 10-fold increase in total yield of enzyme/g of tissue. Homogeneous aryl sulfotransferase IV was used to prepare polyclonal antibodies in male New Zealand White rabbits. Results of immunochemical analyses demonstrated that these antibodies reacted with only a single protein in rat hepatic 100,000 x g supernatant fractions and, further, that the immunoreactive protein had the isoelectric point and subunit molecular mass characteristic of aryl sulfotransferase IV. Immunohistochemical analyses demonstrated that aryl sulfotransferase IV is present in hepatocytes throughout the liver, although centrilobular cells contain a significantly greater (p less than 0.01) amount of aryl sulfotransferase IV than do either midzonal or periportal cells, in which similar levels of the enzyme are found.

Analytical, structural and metabolic biochemistry

Biological and medical sciences

Enzymes and enzyme inhibitors

Fundamental and applied biological sciences. Psychology

Transferases

Details

Title: Subtitle: Purification, immunochemical characterization, and immunohistochemical localization of rat hepatic aryl sulfotransferase IV
Creators: Michael W Duffel - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Thomas P Binder - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Lynn Hosie - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Holly A Baden - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Jill A Sanders - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Shirley A Knapp - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Jeffrey Baron - Univ. Iowa, coll. pharmacy, div. medicinal natural products chemistry, Iowa city IA 52242, United States
Resource Type: Journal article
Publication Details: Molecular pharmacology, Vol.40(1), pp.36-44
Publisher: American Society for Pharmacology and Experimental Therapeutics
DOI: 10.1016/S0026-895X(25)12849-6
PMID: 1857339
ISSN: 0026-895X
eISSN: 1521-0111
Language: English
Date published: 1991
Academic Unit: Pharmaceutical Sciences and Experimental Therapeutics; Neuroscience and Pharmacology; Medicinal and Natural Products Chemistry
Record Identifier: 9984303285502771

Metrics

2 Record Views

26 Times Cited - Web of Science