Journal article
Purification of P-TEFb, a Transcription Factor Required for the Transition into Productive Elongation
The Journal of biological chemistry, Vol.270(21), pp.12335-12338
05/26/1995
DOI: 10.1074/jbc.270.21.12335
PMID: 7759473
Abstract
Production of full-length runoff transcripts in vitro and functional mRNA in vivo is sensitive to the drug 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB). We previously proposed the existence of an activity, P-TEF (positive transcription elongation factor) that functions in a DRB-sensitive manner to allow RNA polymerase II elongation complexes to efficiently synthesize long transcripts (Marshall, N. F. and Price, D. H. (1992) Mol. Cell. Biol. 12, 2078-2090). We have fractionated nuclear extracts of Drosophila melanogaster Kc cells and identified three activities, P-TEFa, factor 2, and P-TEFb, that are directly involved in reconstructing DRB-sensitive transcription. P-TEFb is essential for the production of DRB-sensitive long transcripts in vitro, while P-TEFa and factor 2 are stimulatory. P-TEFb activity is associated with a protein comprising two polypeptide subunits with apparent molecular masses of 124 and 43 kDa. Using a P-TEFb-dependent transcription system, we show that P-TEFb acts after initiation and is the limiting factor in the production of long run-off transcripts.
Details
- Title: Subtitle
- Purification of P-TEFb, a Transcription Factor Required for the Transition into Productive Elongation
- Creators
- Nick F MarshallDavid H Price
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.270(21), pp.12335-12338
- DOI
- 10.1074/jbc.270.21.12335
- PMID
- 7759473
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 05/26/1995
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024532602771
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