Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies

José G Assouline; E. Peter Bosch; Ramon Lim

doi:10.1016/0006-8993(83)90953-8

Back

Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies

Journal article

Peer reviewed

Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies

José G Assouline, E. Peter Bosch and Ramon Lim

Brain research, Vol.277(2), pp.389-392

1983

DOI: 10.1016/0006-8993(83)90953-8

PMID: 6605783

View Online

Abstract

We report a method for deriving purified rat Schwann cells by immunoselective removal of fibroblasts. Contaminating fibroblasts labeled with antibody against specific surface marker Thy 1.1 are bound on plastic surfaces coated with a second antibody. The efficacy of the method is demonstrated by flow cytometry and by specific Schwann cell Ran-1 immunofluorescence.

Cell Culture

panning technique

rat Schwann cell

Details

Title: Subtitle: Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies
Creators: José G Assouline
E. Peter Bosch
Ramon Lim
Resource Type: Journal article
Publication Details: Brain research, Vol.277(2), pp.389-392
Publisher: Elsevier B.V
DOI: 10.1016/0006-8993(83)90953-8
PMID: 6605783
ISSN: 0006-8993
eISSN: 1872-6240
Language: English
Date published: 1983
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Neurology; Iowa Neuroscience Institute; Urology
Record Identifier: 9984065398502771

Metrics

12 Record Views

44 Times Cited - Web of Science