Journal article
Purified monomeric ligand.MD-2 complexes reveal molecular and structural requirements for activation and antagonism of TLR4 by Gram-negative bacterial endotoxins
Immunologic research, Vol.59(1-3), pp.3-11
08/01/2014
DOI: 10.1007/s12026-014-8543-y
PMCID: PMC4125468
PMID: 24895101
Abstract
A major focus of work in our laboratory concerns the molecular mechanisms and structural bases of Gram-negative bacterial endotoxin recognition by host (e.g., human) endotoxin-recognition proteins that mediate and/or regulate activation of Toll-like receptor (TLR) 4. Here, we review studies of wild-type and variant monomeric endotoxin.MD-2 complexes first produced and characterized in our laboratories. These purified complexes have provided unique experimental reagents, revealing both quantitative and qualitative determinants of TLR4 activation and antagonism. This review is dedicated to the memory of Dr. Theresa L. Gioannini (1949-2014) who played a central role in many of the studies and discoveries that are reviewed.
Details
- Title: Subtitle
- Purified monomeric ligand.MD-2 complexes reveal molecular and structural requirements for activation and antagonism of TLR4 by Gram-negative bacterial endotoxins
- Creators
- Theresa L. Gioannini - University of IowaAthmane Teghanemt - University of IowaDeSheng Zhang - University of IowaGregory Esparza - University of IowaLiping Yu - University of IowaJerrold Weiss - United States Department of Veterans Affairs
- Resource Type
- Journal article
- Publication Details
- Immunologic research, Vol.59(1-3), pp.3-11
- DOI
- 10.1007/s12026-014-8543-y
- PMID
- 24895101
- PMCID
- PMC4125468
- NLM abbreviation
- Immunol Res
- ISSN
- 0257-277X
- eISSN
- 1559-0755
- Publisher
- Humana Press Inc
- Number of pages
- 9
- Grant note
- AI059372; AI088372; AI044642 / National Institute of Allergy and Infectious Disease, National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01AI088372 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) 1I01BX0000949-01A1 / Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development grant I01BX000949 / Veterans Affairs; US Department of Veterans Affairs
- Language
- English
- Date published
- 08/01/2014
- Academic Unit
- Infectious Diseases; Biochemistry and Molecular Biology; Medicine Administration; Internal Medicine
- Record Identifier
- 9984627286502771
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