Journal article
Purinosome formation as a function of the cell cycle
Proceedings of the National Academy of Sciences - PNAS, Vol.112(5), pp.1368-1373
02/03/2015
DOI: 10.1073/pnas.1423009112
PMCID: PMC4321313
PMID: 25605889
Abstract
The de novo purine biosynthetic pathway relies on six enzymes to catalyze the conversion of phosphoribosylpyrophosphate to inosine 5'-monophosphate. Under purine-depleted conditions, these enzymes form a multienzyme complex known as the purinosome. Previous studies have revealed the spatial organization and importance of the purinosome within mammalian cancer cells. In this study, time-lapse fluorescence microscopy was used to investigate the cell cycle dependency on purinosome formation in two cell models. Results in HeLa cells under purine-depleted conditions demonstrated a significantly higher number of cells with purinosomes in the G(1) phase, which was further confirmed by cell synchronization. HGPRT-deficient fibroblast cells also exhibited the greatest purinosome formation in the G(1) phase; however, elevated levels of purinosomes were also observed in the S and G(2)/M phases. The observed variation in cell cycle-dependent purinosome formation between the two cell models tested can be attributed to differences in purine biosynthetic mechanisms. Our results demonstrate that purinosome formation is closely related to the cell cycle.
Details
- Title: Subtitle
- Purinosome formation as a function of the cell cycle
- Creators
- Chung Yu Chan - Pennsylvania State UniversityHong Zhao - Pennsylvania State UniversityRaymond J. Pugh - Pennsylvania State UniversityAnthony M. Pedley - Pennsylvania State UniversityJarrod French - Stony Brook UniversitySara A. Jones - Harvard UniversityXiaowei Zhuang - Harvard UniversityHyder Jinnah - Emory UniversityTony Jun Huang - Pennsylvania State UniversityStephen J. Benkovic - Pennsylvania State University
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.112(5), pp.1368-1373
- DOI
- 10.1073/pnas.1423009112
- PMID
- 25605889
- PMCID
- PMC4321313
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- Natl Acad Sciences
- Number of pages
- 6
- Grant note
- GM024129 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01GM024129 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Language
- English
- Date published
- 02/03/2015
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984771644402771
Metrics
6 Record Views