Journal article
Putative Stem Cells in Human Dental Pulp with Irreversible Pulpitis: An Exploratory Study
Journal of endodontics, Vol.36(5), pp.820-825
05/2010
DOI: 10.1016/j.joen.2010.02.003
PMCID: PMC2907071
PMID: 20416426
Abstract
Although human dental pulp stem cells isolated from healthy teeth have been extensively characterized, it is unknown whether stem cells also exist in clinically compromised teeth with irreversible pulpitis. Here we explored whether cells retrieved from clinically compromised dental pulp have stem cell–like properties.\nPulp cells were isolated from healthy teeth (control group) and from teeth with clinically diagnosed irreversible pulpitis (diseased group). Cell proliferation, stem cell marker STRO-1 expression, and cell odonto-osteogenic differentiation competence were compared.\nCells from the diseased group demonstrated decreased colony formation capacity and a slightly decreased cell proliferation rate, but they had similar STRO-1 expression and exhibited a similar percentage of positive ex vivo osteogenic induction and dentin sialophosphoprotein expression from STRO-1–enriched pulp cells.\nOur study provides preliminary evidence that clinically compromised dental pulp might contain putative cells with certain stem cell properties. Further characterization of these cells will provide insight regarding whether they could serve as a source of endogenous multipotent cells in tissue regeneration–based dental pulp therapy.
Details
- Title: Subtitle
- Putative Stem Cells in Human Dental Pulp with Irreversible Pulpitis: An Exploratory Study
- Creators
- Zhengyan Wang - Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaJian Pan - Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaJohn T Wright - Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaSompop Bencharit - Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaShaoping Zhang - Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaEric T Everett - Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaFabricio B Teixeira - Department of Endodontics, University of North Carolina at Chapel Hill, Chapel Hill, North CarolinaJohn S Preisser - Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
- Resource Type
- Journal article
- Publication Details
- Journal of endodontics, Vol.36(5), pp.820-825
- DOI
- 10.1016/j.joen.2010.02.003
- PMID
- 20416426
- PMCID
- PMC2907071
- NLM abbreviation
- J Endod
- ISSN
- 0099-2399
- eISSN
- 1878-3554
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 05/2010
- Academic Unit
- Endodontics; Periodontics
- Record Identifier
- 9984065999502771
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