Journal article
Pyelonephritogenic Escherichia coli and killing of cultured human renal proximal tubular epithelial cells: role of hemolysin in some strains
Infection and immunity, Vol.58(5), pp.1281-1289
05/1990
DOI: 10.1128/IAI.58.5.1281-1289.1990
PMCID: PMC258621
PMID: 2182540
Abstract
Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate dehydrogenase (LDH). Thirty-four isolates cultured from patients with acute pyelonephritis were screened for the ability to cause pyelonephritis in CBA mice by transurethral challenge. The eight most virulent strains (greater than or equal to 70% of mice challenged developed greater than or equal to 10(3) CFU/g of kidney after 48 h) were selected for study. Each strain displayed mannose-resistant hemagglutination of human O erythrocytes; three strains were phenotypically and genotypically hemolytic. Pyelonephritogenic strains were significantly more cytotoxic (30.1 +/- 9.5% LDH release after 18 h) than eight fecal control strains (13.5 +/- 11.5% LDH release; P = 0.0068). We selected the most cytotoxic strain, CFT073, for further study. Sterile filtrate from this hemolytic strain was significantly more cytotoxic than was the filtrate of the fecal control strain, FN414. Transposon mutagenesis of CFT073 with TnphoA abolished hemolytic activity and cytotoxicity by both whole cells and sterile filtrate. Southern blot analysis revealed that the Tnphoa insertion mapped to the E. coli chromosomal hly determinant within a 12-kilobase SalI restriction fragment. Transformation of a nonhemolytic strain, CPZ005 with plasmid pSF4000, which carries a cloned hemolysin determinant, resulted in highly elevated cytotoxicity. Light micrographs of proximal tubular epithelial cell cultures demonstrated cell damage by pyelonephritogenic strains that was not induced by a fecal strain or the hemolysin-deficient mutant. Results indicate that pyelonephritogenic E. coli strains are more frequently cytotoxic for a putative target, that is, human renal tubular epithelium, than are fecal isolates. Hemolysin, in some strains, is apparently responsible for this cytotoxicity.
Details
- Title: Subtitle
- Pyelonephritogenic Escherichia coli and killing of cultured human renal proximal tubular epithelial cells: role of hemolysin in some strains
- Creators
- Harry L T Mobley - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201Deborah M Green - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201Anna L Trifillis - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201David E Johnson - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201Gwynn R Chippendale - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201C Virginia Lockatell - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201Bradley D Jones - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201John W Warren - Department of Medicine, University of Maryland School of Medicine, Baltimore 21201
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.58(5), pp.1281-1289
- DOI
- 10.1128/IAI.58.5.1281-1289.1990
- PMID
- 2182540
- PMCID
- PMC258621
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 05/1990
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083809802771
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