QRX, a novel homeobox gene, modulates photoreceptor gene expression

Qing-liang Wang; Shiming Chen; Noriko Esumi; Prabodh K Swain; Heidi S Haines; Guanghua Peng; B Michele Melia; Iain McIntosh; John R Heckenlively; Samuel G Jacobson; Edwin M Stone; Anand Swaroop; Donald J Zack

doi:10.1093/hmg/ddh117

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QRX, a novel homeobox gene, modulates photoreceptor gene expression

Journal article

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Peer reviewed

QRX, a novel homeobox gene, modulates photoreceptor gene expression

Qing-liang Wang, Shiming Chen, Noriko Esumi, Prabodh K Swain, Heidi S Haines, Guanghua Peng, B Michele Melia, Iain McIntosh, John R Heckenlively, Samuel G Jacobson, …

Human molecular genetics, Vol.13(10), pp.1025-1040

05/15/2004

DOI: 10.1093/hmg/ddh117

PMID: 15028672

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Abstract

A novel paired-like homeobox gene, designated as Qrx, was identified by a yeast one-hybrid screen using the bovine Rhodopsin promoter Ret-1 DNA regulatory element as bait. Qrx is preferentially expressed in both the outer and inner nuclear layers of the retina. Its homeodomain is nearly identical to that of Rx/Rax, a transcription factor that is essential for eye development, but it shares only limited homology elsewhere. Although Qrx and Rx/Rax show similar DNA binding properties in vitro, the two proteins demonstrate distinct target selectivity and functional behavior in promoter activity assays. QRX synergistically increases the transactivating function of the photoreceptor transcription factors Crx and NRL and it physically interacts with CRX. Qrx is present in the bovine and human genomes, but appears to be absent from the mouse genome. Nonetheless, a 5.8 kb upstream region of human QRX is capable of directing expression in presumptive photoreceptor precursor cells in transgenic mice. These results indicate that Qrx may be involved in modulating photoreceptor gene expression. In addition, the finding of rare heterozygous QRX sequence changes in three individuals with retinal degeneration raises the possibility that QRX may be involved in disease pathogenesis.

Retinal Diseases - genetics

Homeodomain Proteins - metabolism

Humans

Molecular Sequence Data

Genes, Homeobox - genetics

Coatomer Protein - genetics

Promoter Regions, Genetic - genetics

DNA-Binding Proteins - metabolism

Chromosomes, Human, Pair 9 - genetics

Point Mutation - genetics

Cattle

Trans-Activators - genetics

Response Elements - genetics

Eye Proteins - genetics

Amino Acid Sequence

Gene Expression Regulation - genetics

Chromosome Mapping

Transcription Factors - genetics

DNA-Binding Proteins - genetics

Homeodomain Proteins - genetics

DNA Footprinting

Transcription Factors - metabolism

Sequence Alignment

Animals

Rhodopsin - genetics

Eye Proteins - metabolism

Protein Binding

Trans-Activators - metabolism

Basic-Leucine Zipper Transcription Factors

Details

Title: Subtitle: QRX, a novel homeobox gene, modulates photoreceptor gene expression
Creators: Qing-liang Wang - Johns Hopkins University School of Medicine
Shiming Chen
Noriko Esumi
Prabodh K Swain
Heidi S Haines
Guanghua Peng
B Michele Melia
Iain McIntosh
John R Heckenlively
Samuel G Jacobson
Edwin M Stone
Anand Swaroop
Donald J Zack
Resource Type: Journal article
Publication Details: Human molecular genetics, Vol.13(10), pp.1025-1040
DOI: 10.1093/hmg/ddh117
PMID: 15028672
NLM abbreviation: Hum Mol Genet
ISSN: 0964-6906
eISSN: 1460-2083
Publisher: England
Grant note: R01 EY012543 / NEI NIH HHS F32 EY006614 / NEI NIH HHS
Language: English
Date published: 05/15/2004
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983980293702771

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