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Quality assurance and use of PCR in clinical trials
Journal article   Open access   Peer reviewed

Quality assurance and use of PCR in clinical trials

P S Reichelderfer and J B Jackson
PCR methods and applications, Vol.4(3), pp.S141-S149
12/1994
DOI: 10.1101/gr.4.3.S141
PMID: 7580896
url
https://doi.org/10.1101/gr.4.3.S141View
Published (Version of record) Open Access

Abstract

Qualitative and quantitative HIV-1 DNA and RNA PCR assays are proving to be useful in the diagnosis of HIV-1 infection in infants and in assessing the in vivo antiviral activity of new therapies and regimens in clinical trials. The use of these standardized commercial assays in conjunction with an external quality assurance program has ensured that results from different laboratories are comparable. In addition, real-time proficiency monitoring has the potential to detect problems immediately before patient data are compromised.
HIV Infections - blood Guidelines as Topic Pregnancy Complications, Infectious - virology Humans Clinical Trials as Topic Infectious Disease Transmission, Vertical HIV-1 - genetics DNA, Viral - blood Pregnancy Lymphocytes - virology HIV-1 - isolation & purification HIV Infections - diagnosis Polymerase Chain Reaction - standards Female HIV Infections - transmission Infant, Newborn Quality Control

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