Journal article
Quantification and Interpretation of Nitric Oxide-Dependent Cutaneous Vasodilation During Local Heating
Journal of applied physiology (1985), Vol.137(5), pp.1418-1424
11/01/2024
DOI: 10.1152/japplphysiol.00558.2024
PMCID: PMC11573251
PMID: 39417818
Abstract
Human cutaneous microdialysis approaches for assessing nitric oxide (NO)-dependent blood flow include local heating (LH) of the skin until a plateau is reached, followed by infusion of a NO synthase inhibitor such as L-NAME; however, varied methods of quantifying and expressing NO-dependent vasodilation can obfuscate data interpretation and reproducibility. We retrospectively assessed NO-dependent vasodilation during LH to 39°C or 42°C, calculated as the: (1) absolute contribution of the NO-dependent component (along with baseline and the non-NO-dependent component) to the total cutaneous vascular conductance (CVC) response to LH, normalized to maximal CVC (%CVC
); (2) difference in %CVC
(∆%CVC
) between the LH plateau and post-NO inhibition (L-NAME plateau; ∆%CVC
=LH plateau-L-NAME plateau); (3) percentage of the LH plateau attributable to ∆%CVC
(%Plateau = ∆%CVC
/LH plateau*100); and (4) %Plateau when correcting for baseline. The LH plateaus during 39°C and 42°C were 48±17 %CVC
(9±5% baseline; 2±4% non-NO-dependent; 36±15% NO-dependent) and 88±10 %CVC
(15±8% baseline; 9±10% non-NO-dependent; 64±13% NO-dependent), respectively. The absolute contributions of the non-NO-dependent and NO-dependent components of the response (p<0.0001) and the ∆%CVC
(66±14% vs. 38±15%) were greater during 42°C compared with 39°C (all p≤0.02); however, there were no differences between the two protocols in %Plateau (75±13% vs. 80±10%; p=0.57) or %Plateau
(88±14% vs. 95±8%; p=0.31). For both protocols, the values were greater for %Plateau
versus ∆%CVC
and %Plateau (p≤0.0001), and for %Plateau versus ∆%CVC
(p≤0.05). Quantification of NO-dependent skin vasodilation responses to LH is dependent upon the mathematical approach and verbal description, which can meaningfully impact data interpretation and reproducibility.
Details
- Title: Subtitle
- Quantification and Interpretation of Nitric Oxide-Dependent Cutaneous Vasodilation During Local Heating
- Creators
- S Tony Wolf - University of GeorgiaGabrielle A Dillon - Mayo Clinic in ArizonaLacy M Alexander - Pennsylvania State UniversityW Larry Kenney - Pennsylvania State UniversityAnna E Stanhewicz - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.137(5), pp.1418-1424
- DOI
- 10.1152/japplphysiol.00558.2024
- PMID
- 39417818
- PMCID
- PMC11573251
- NLM abbreviation
- J Appl Physiol (1985)
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Publisher
- AMER PHYSIOLOGICAL SOC
- Grant note
- P30 ES005605 / NIEHS NIH HHS Penn State Center for Human Evolution and Diversity 5T32AG049676-03 / HHS | National Institutes of Health (NIH) CDA 937990 / American Heart Association (AHA) R01 AG067471 / NIA NIH HHS UL1TR002537 / HHS | National Institutes of Health (NIH)
- Language
- English
- Electronic publication date
- 10/17/2024
- Date published
- 11/01/2024
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984737129702771
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