Quantification of Cefepime, Meropenem, Piperacillin, and Tazobactam in Human Plasma Using a Sensitive and Robust Liquid Chromatography-Tandem Mass Spectrometry Method, Part 2: Stability Evaluation

Ronilda D'Cunha; Thanh Bach; Beth Ann Young; Peizhi Li; Demet Nalbant; Jun Zhang; Patricia Winokur; Guohua An

doi:10.1128/AAC.00861-18

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Quantification of Cefepime, Meropenem, Piperacillin, and Tazobactam in Human Plasma Using a Sensitive and Robust Liquid Chromatography-Tandem Mass Spectrometry Method, Part 2: Stability Evaluation

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Quantification of Cefepime, Meropenem, Piperacillin, and Tazobactam in Human Plasma Using a Sensitive and Robust Liquid Chromatography-Tandem Mass Spectrometry Method, Part 2: Stability Evaluation

Ronilda D'Cunha, Thanh Bach, Beth Ann Young, Peizhi Li, Demet Nalbant, Jun Zhang, Patricia Winokur and Guohua An

Antimicrobial agents and chemotherapy, Vol.62(9), e00861-18

08/27/2018

DOI: 10.1128/AAC.00861-18

PMCID: PMC6125492

PMID: 29941653

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Abstract

Although the stability of β-lactam antibiotics is a known issue, none of the previously reported bioanalytical methods had an adequate evaluation of the stability of these drugs. In the current study, the stability of cefepime, meropenem, piperacillin, and tazobactam under various conditions was comprehensively evaluated. The evaluated parameters included stock solution stability, short-term stability, long-term stability, freeze-thaw stability, processed sample stability, and whole-blood stability. When stored at −20°C, the stock solution of meropenem in methanol was stable for up to 3 weeks, and the stock solutions of cefepime, piperacillin, and tazobactam were stable for up to 6 weeks. All four antibiotics were stable in human plasma for up to 3 months when stored at −80°C and were stable in whole blood for up to 4 h at room temperature. Short-term stability results indicated that all four β-lactams were stable at room temperature for 2 h, but substantial degradation was observed when the plasma samples were stored at room temperature for 24 h, with the degradation rates for cefepime, meropenem, piperacillin, and tazobactam being 30.1%, 75.6%, 49.0%, and 37.7%, respectively. Because the stability information is method independent, our stability results can be used as a reference by other research groups that work with these antibiotics.

Analytical Procedures

Anti-Bacterial Agents

Cefepime

Comment On

Meropenem

Piperacillin

Tazobactam

Details

Title: Subtitle: Quantification of Cefepime, Meropenem, Piperacillin, and Tazobactam in Human Plasma Using a Sensitive and Robust Liquid Chromatography-Tandem Mass Spectrometry Method, Part 2: Stability Evaluation
Creators: Ronilda D'Cunha - University of Iowa
Thanh Bach - University of Iowa
Beth Ann Young - University of Iowa
Peizhi Li - University of Iowa
Demet Nalbant - University of Iowa
Jun Zhang - Dynamega, Lake Forest, Illinois, USA.
Patricia Winokur - University of Iowa
Guohua An - University of Iowa
Resource Type: Journal article
Publication Details: Antimicrobial agents and chemotherapy, Vol.62(9), e00861-18
DOI: 10.1128/AAC.00861-18
PMID: 29941653
PMCID: PMC6125492
NLM abbreviation: Antimicrob Agents Chemother
ISSN: 0066-4804
eISSN: 1098-6596
Publisher: American Society for Microbiology
Number of pages: 9
Grant note: HHSN272200800008C / HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) (https://doi.org/10.13039/100000060)
Alternative title: Quantification of β-Lactam Antibiotics in Human Plasma, D'Cunha et al
Language: English
Date published: 08/27/2018
Academic Unit: Infectious Diseases; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Medicine Administration; Internal Medicine
Record Identifier: 9984359891002771

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