Quantification of repertoire diversity of influenza-specific epitopes with predominant public or private TCR usage

Katherine Kedzierska; E Bridie Day; Jing Pi; Stephen B Heard; Peter C Doherty; Stephen J Turner; Stanley Perlman

doi:10.4049/jimmunol.177.10.6705

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Quantification of repertoire diversity of influenza-specific epitopes with predominant public or private TCR usage

Journal article

Peer reviewed

Quantification of repertoire diversity of influenza-specific epitopes with predominant public or private TCR usage

Katherine Kedzierska, E Bridie Day, Jing Pi, Stephen B Heard, Peter C Doherty, Stephen J Turner and Stanley Perlman

Journal of immunology (Baltimore, Md. : 1950), Vol.177(10), pp.6705-6712

11/15/2006

DOI: 10.4049/jimmunol.177.10.6705

PMID: 17082583

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Abstract

The H-2Db-restricted CD8 T cell immune response to influenza A is directed at two well-described epitopes, nucleoprotein 366 (NP366) and acid polymerase 224 (PA224). The responses to the two epitopes are very different. The epitope NP366-specific response is dominated by TCR clonotypes that are public (shared by most mice), whereas the epitope PA224-specific response is private (unique within each infected animal). In addition to being public, the NP366-specific response is dominated by a few clonotypes, when T cell clonotypes expressing the Vbeta8.3 element are analyzed. Herein, we show that this response is similarly public when the NP366+Vbeta4+ CD8 T cell response is analyzed. Furthermore, to determine whether these features resulted in differences in total TCR diversity in the NP366+ and PA224+ responses, we quantified the number of different CD8 T clonotypes responding to each epitope. We calculated that 50-550 clonotypes recognized each epitope in individual mice. Thus, although the character of the response to the two epitopes appeared to be different (private and diverse vs public and dominated by a few clonotypes), similar numbers of precursor cells responded to both epitopes and this number was of similar magnitude to that previously reported for other viral CD8 T cell epitopes. Therefore, even in CD8 T cell responses that appear to be oligoclonotypic, the total response is highly diverse.

Lymphocytic choriomeningitis virus - immunology

Cell Line

Epitopes, T-Lymphocyte - biosynthesis

Influenza A Virus, H3N2 Subtype - enzymology

Epitopes, T-Lymphocyte - analysis

Mice, Inbred C57BL

RNA-Binding Proteins - immunology

Influenza A Virus, H3N2 Subtype - immunology

CD8-Positive T-Lymphocytes - enzymology

Murine hepatitis virus - immunology

Animals

CD8-Positive T-Lymphocytes - virology

Receptors, Antigen, T-Cell, alpha-beta - biosynthesis

Dogs

CD8-Positive T-Lymphocytes - metabolism

Female

Viral Core Proteins - immunology

Clone Cells

Mice

Receptors, Antigen, T-Cell, alpha-beta - metabolism

CD8-Positive T-Lymphocytes - immunology

Epitopes, T-Lymphocyte - metabolism

RNA Replicase - immunology

Nucleoproteins - immunology

Details

Title: Subtitle: Quantification of repertoire diversity of influenza-specific epitopes with predominant public or private TCR usage
Creators: Katherine Kedzierska - Department of Microbiology and Immunology, The University of Melbourne, Parkeville, Victoria, Australia
E Bridie Day
Jing Pi
Stephen B Heard
Peter C Doherty
Stephen J Turner
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of immunology (Baltimore, Md. : 1950), Vol.177(10), pp.6705-6712
DOI: 10.4049/jimmunol.177.10.6705
PMID: 17082583
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: NS036592 / NINDS NIH HHS
Language: English
Date published: 11/15/2006
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777345202771

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