Journal article
Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates
Methods in molecular biology (Clifton, N.J.), Vol.1427, pp.305-318
2016
DOI: 10.1007/978-1-4939-3615-1_18
PMCID: PMC5258114
PMID: 27259935
Abstract
The first successful in vitro experiments on the cochlea were conducted in 1928 by Honor Fell (Fell, Arch Exp Zellforsch 7(1):69-81, 1928). Since then, techniques for culture of this tissue have been refined, and dissociated primary culture of the spiral ganglion has become a widely accepted in vitro model for studying nerve damage and regeneration in the cochlea. Additionally, patterned substrates have been developed that facilitate and direct neural outgrowth. A number of automated and semi-automated methods for quantifying this neurite outgrowth have been utilized in recent years (Zhang et al., J Neurosci Methods 160(1):149-162, 2007; Tapias et al., Neurobiol Dis 54:158-168, 2013). Here, we describe a method to study the effect of topographical cues on spiral ganglion neurite and Schwann cell alignment. We discuss our microfabrication process, characterization of pattern features, cell culture techniques for both spiral ganglion neurons and spiral ganglion Schwann cells. In addition, we describe protocols for reducing fibroblast count, immunocytochemistry, and methods for quantifying neurite and Schwann cell alignment.
Details
- Title: Subtitle
- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates
- Creators
- Elise L Cheng - Department of Otolaryngology-Head and Neck Surgery, University of Iowa Hospitals and Clinics, Iowa City, IA, USABraden Leigh - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA, USAC Allan Guymon - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA, USAMarlan R Hansen - Department of Neurosurgery, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, Iowa City, IA, 52242, USA. marlan-hansen@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1427, pp.305-318
- DOI
- 10.1007/978-1-4939-3615-1_18
- PMID
- 27259935
- PMCID
- PMC5258114
- NLM abbreviation
- Methods Mol Biol
- eISBN
- 9781493936151; 1493936158
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Publisher
- United States
- Grant note
- R01 DC012578 / NIDCD NIH HHS
- Language
- English
- Date published
- 2016
- Academic Unit
- Molecular Physiology and Biophysics; Chemical and Biochemical Engineering; Neurosurgery; Otolaryngology
- Record Identifier
- 9984006367502771
Metrics
27 Record Views