Journal article
Quantifying proteomes and their post-translational modifications by stable isotope label-based mass spectrometry
Current opinion in chemical biology, Vol.17(5), pp.779-786
10/2013
DOI: 10.1016/j.cbpa.2013.06.011
PMCID: PMC3823833
PMID: 23835517
Abstract
•Stable isotopes enable accurate and multiplexed proteome quantification by MS.•Quantitative strategies can elucidate physical interactions and signaling networks.•Integrative approaches profile multiple stages of gene expression regulation.•Quantitative MS is becoming increasingly equipped to impact translational science.
Stable isotope labeling coupled with mass spectrometry has revolutionized the scope and impact of protein expression studies. Label incorporation can occur metabolically or chemically, and each method bears specific strengths and weaknesses. Quantitative proteomics confidently identifies specific interactions between proteins and other biological species, such as nucleic acids and metabolites. Extending label-based methods to phosphorylation-modified forms of proteins enables the construction of signaling networks and their temporal responses to stimuli. The integration of multiple data types offers systems-level insight on coordinated biological processes. Finally, the development of methods applicable to tissue quantification suggests the emerging role of label-based, quantitative mass spectrometry in translational science.
Details
- Title: Subtitle
- Quantifying proteomes and their post-translational modifications by stable isotope label-based mass spectrometry
- Creators
- Anna E Merrill - Department of Chemistry, University of Wisconsin, 1101 University Avenue, Madison, WI 53706, United StatesJoshua J Coon - Department of Chemistry, University of Wisconsin, 1101 University Avenue, Madison, WI 53706, United States
- Resource Type
- Journal article
- Publication Details
- Current opinion in chemical biology, Vol.17(5), pp.779-786
- DOI
- 10.1016/j.cbpa.2013.06.011
- PMID
- 23835517
- PMCID
- PMC3823833
- NLM abbreviation
- Curr Opin Chem Biol
- ISSN
- 1367-5931
- eISSN
- 1879-0402
- Publisher
- Elsevier BV
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01 GM080148; name: National Institutes of Health-funded Genomic Sciences Training Program, award: 5T32HG002760
- Language
- English
- Date published
- 10/2013
- Academic Unit
- Pathology; Injury Prevention Research Center
- Record Identifier
- 9984047784602771
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