Quantitation of CD8+ T cell expansion, memory, and protective immunity after immunization with peptide-coated dendritic cells

Sara E Hamilton; John T Harty

doi:10.4049/jimmunol.169.9.4936

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Quantitation of CD8+ T cell expansion, memory, and protective immunity after immunization with peptide-coated dendritic cells

Journal article

Peer reviewed

Quantitation of CD8+ T cell expansion, memory, and protective immunity after immunization with peptide-coated dendritic cells

Sara E Hamilton and John T Harty

The Journal of immunology (1950), Vol.169(9), pp.4936-4944

11/01/2002

DOI: 10.4049/jimmunol.169.9.4936

PMID: 12391206

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Abstract

Dendritic cells (DCs) are potent APCs for naive CD8(+) T cells and are being investigated as vaccine delivery vehicles. In this study, we examine the CD8(+) T cell response to defined peptides from Listeria monocytogenes (LM), lymphocytic choriomeningitis virus, and murine CMV coated singly and in combination onto mature bone marrow-derived DCs (BMDCs). We show that immunization of mice with 2 x 10(5) mature BMDCs coated with multiple MHC class I peptides generates a significant Ag-specific CD8(+) T cell response in both the spleen and nonlymphoid organs. This immunization resulted in a peptide-specific hierarchy in the magnitude of CD8(+) T cell priming and noncoordinate kinetics in response to different peptide epitopes. Kinetics were not exclusively due to specific characteristics of the MHC class I molecule, and were not altered in an Ag-independent manner by concurrent LM infection. Mice immunized with listeriolysin O 91-99-coated BMDCs are protected against high dose challenge with virulent LM. This protection was enhanced by diversifying the memory CD8(+) T cell compartment, even in the absence of a large increase in Ag-specific CD8(+) memory T cells.

CD8-Positive T-Lymphocytes - cytology

Lung - microbiology

Injections, Intravenous

Dendritic Cells - immunology

Liver - microbiology

Histocompatibility Antigen H-2D

Listeriosis - prevention & control

Lung - cytology

Liver - immunology

Antigen Presentation

Bacterial Toxins

Cell Division - immunology

Bone Marrow Cells - immunology

Bone Marrow Transplantation

Listeriosis - immunology

Female

Bacterial Proteins - immunology

Epitopes, T-Lymphocyte - immunology

CD8-Positive T-Lymphocytes - microbiology

Heat-Shock Proteins - administration & dosage

Epitopes, T-Lymphocyte - analysis

H-2 Antigens - metabolism

Hemolysin Proteins

Lymphocyte Activation

Heat-Shock Proteins - metabolism

Oligopeptides - immunology

Oligopeptides - metabolism

Immunity, Innate

Bacterial Proteins - administration & dosage

Heat-Shock Proteins - immunology

H-2 Antigens - immunology

Animals

Bone Marrow Cells - microbiology

Dendritic Cells - microbiology

Lymphocyte Count

Bacterial Proteins - metabolism

Immunologic Memory

Liver - cytology

Mice

Mice, Inbred BALB C

Oligopeptides - administration & dosage

Kinetics

CD8-Positive T-Lymphocytes - immunology

Dendritic Cells - transplantation

Lung - immunology

Details

Title: Subtitle: Quantitation of CD8+ T cell expansion, memory, and protective immunity after immunization with peptide-coated dendritic cells
Creators: Sara E Hamilton - Interdisciplinary Program in Immunology and Department of Microbiology, University of Iowa, Iowa City 52242, USA
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.169(9), pp.4936-4944
DOI: 10.4049/jimmunol.169.9.4936
PMID: 12391206
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: AI42767 / NIAID NIH HHS AI46653 / NIAID NIH HHS AI50073 / NIAID NIH HHS
Language: English
Date published: 11/01/2002
Academic Unit: Pathology
Record Identifier: 9984046818902771

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