Journal article
Quantitative 3-D morphometric analysis of individual dendritic spines
Scientific reports, Vol.8(1), 3545
02/23/2018
DOI: 10.1038/s41598-018-21753-8
PMCID: PMC5825014
PMID: 29476060
Abstract
The observation and analysis of dendritic spines morphological changes poses a major challenge in neuroscience studies. The alterations of their density and/or morphology are indicators of the cellular processes involved in neural plasticity underlying learning and memory, and are symptomatic in neuropsychiatric disorders. Despite ongoing intense investigations in imaging approaches, the relationship between changes in spine morphology and synaptic function is still unknown. The existing quantitative analyses are difficult to perform and require extensive user intervention. Here, we propose a new method for (1) the three-dimensional (3-D) segmentation of dendritic spines using a multi-scale opening approach and (2) define 3-D morphological attributes of individual spines for the effective assessment of their structural plasticity. The method was validated using confocal light microscopy images of dendritic spines from dissociated hippocampal cultures and brain slices (1) to evaluate accuracy relative to manually labeled ground-truth annotations and relative to the state-of-the-art Imaris tool, (2) to analyze reproducibility of user-independence of the segmentation method, and (3) to quantitatively analyze morphological changes in individual spines before and after chemically induced long-term potentiation. The method was monitored and used to precisely describe the morphology of individual spines in real-time using consecutive images of the same dendritic fragment.
Details
- Title: Subtitle
- Quantitative 3-D morphometric analysis of individual dendritic spines
- Creators
- Subhadip Basu - Department of Computer Science and Engineering, Jadavpur University, Kolkata, 700032, India. subhadip@cse.jdvu.ac.inPunam Kumar Saha - Department of Electrical and Computer Engineering and Department of Radiology, University of Iowa, Iowa City, IA, 52242, USAMatylda Roszkowska - Nencki Institute of Experimental Biology, Polish Academy of Sciences, Pasteura 3, Warsaw, 02-093, PolandMarta Magnowska - Nencki Institute of Experimental Biology, Polish Academy of Sciences, Pasteura 3, Warsaw, 02-093, PolandEwa Baczynska - Nencki Institute of Experimental Biology, Polish Academy of Sciences, Pasteura 3, Warsaw, 02-093, PolandNirmal Das - Department of Computer Science and Engineering, Jadavpur University, Kolkata, 700032, IndiaDariusz Plewczynski - Center of New Technologies, University of Warsaw, Banacha 2c Street, Warsaw, 02-097, PolandJakub Wlodarczyk - Nencki Institute of Experimental Biology, Polish Academy of Sciences, Pasteura 3, Warsaw, 02-093, Poland. j.wlodarczyk@nencki.gov.pl
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.8(1), 3545
- DOI
- 10.1038/s41598-018-21753-8
- PMID
- 29476060
- PMCID
- PMC5825014
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- England
- Grant note
- U54 DK107967 / NIDDK NIH HHS
- Language
- English
- Date published
- 02/23/2018
- Academic Unit
- Radiology; Electrical and Computer Engineering
- Record Identifier
- 9984051521402771
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