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Quantitative Analysis of Retinal Layer Optical Intensities on Three-Dimensional Optical Coherence Tomography
Journal article   Open access   Peer reviewed

Quantitative Analysis of Retinal Layer Optical Intensities on Three-Dimensional Optical Coherence Tomography

Xinjian Chen, Ping Hou, Chao Jin, Weifang Zhu, Xiaohong Luo, Fei Shi, Milan Sonka and Haoyu Chen
Investigative ophthalmology & visual science, Vol.54(10), pp.6846-6851
10/21/2013
DOI: 10.1167/iovs.13-12062
PMCID: PMC5963175
PMID: 24045992
url
https://doi.org/10.1167/iovs.13-12062View
Published (Version of record) Open Access

Abstract

Purpose.: To investigate the optical intensities of all retinal layers on three-dimensional (3D) spectral-domain optical coherence tomography (SD-OCT) in normal subjects using an automatic measurement. Methods.: Forty normal subjects underwent Topcon 3D OCT-1000 macula-centered scan. The raw data were automatically segmented into 10 layers using the 3D graph search approach. Then the mean and standard deviation of intensities of each layer were calculated. The image quality index was given by the OCT software. Correlation analysis was performed between the optical intensities in each layer and image quality and subject's age. Results.: The correlation of optical intensities was strong from ganglion cell layer (GCL) to outer nuclear layer (ONL) with r > 0.934; moderate among retinal nerve fiber layer (RNFL), photoreceptor, retinal pigment epithelium (RPE), and choroid (0.410 < r < 0.800); and low in the vitreous (0.251 < r < 0.541). The optical intensities were also correlated with the image quality, r > 0.869 from GCL to ONL, 0.748 < r < 0.802 for RNFL, photoreceptor layer, RPE, and the choroid, r = 0.528 for the vitreous. The optical intensity in RNFL was negatively correlated with age (r = −0.365). Conclusions.: Automatic assessment of the layers' intensities was achieved. In normal subjects, the retinal layers' optical intensities were affected by image quality. Normalization with optical intensity of ONL, all areas, or image quality index is recommended. The optical intensity of RNFL decreased with age.

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