Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in a Murine Asthma Model

Zachary Hutchins; Xingyuan Chen; Abu Eabrahim Siddique; Peter S Thorne; Aliasger K Salem; Josalyn L Cho; Andrea Adamcakova-Dodd; Yinsheng Wang

doi:10.1021/acs.chemrestox.6c00094

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Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in a Murine Asthma Model

Journal article

Peer reviewed

Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in a Murine Asthma Model

Zachary Hutchins, Xingyuan Chen, Abu Eabrahim Siddique, Peter S Thorne, Aliasger K Salem, Josalyn L Cho, Andrea Adamcakova-Dodd and Yinsheng Wang

Chemical research in toxicology

04/09/2026

DOI: 10.1021/acs.chemrestox.6c00094

PMID: 41958033

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Abstract

Extracellular vesicles (EVs) are key mediators of intercellular communication and immune regulation; however, their proteomic composition in allergic asthma remains poorly understood. We performed tandem mass tag (TMT)-based quantitative proteomic analyses of bronchoalveolar lavage fluid (BALF) and BALF-derived EVs in a murine house dust mite (HDM) model of allergic airway inflammation. HDM challenge elicited significant upregulation of Th2-associated proteins (CLCA1, FCGBP, CHIL3, CHIL4, and RETNLA), which is consistent with hallmark features of asthma. EPX and CKM were detected exclusively in BALF-derived EVs, demonstrating that EVs can selectively carry disease-relevant proteins. Our results underscore EV's cargo-mediated mechanisms in asthma pathogenesis.

Details

Title: Subtitle: Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in a Murine Asthma Model
Creators: Zachary Hutchins - University of California System
Xingyuan Chen - University of California System
Abu Eabrahim Siddique - University of Iowa
Peter S Thorne - University of Iowa
Aliasger K Salem - University of Iowa
Josalyn L Cho - University of Iowa
Andrea Adamcakova-Dodd - University of Iowa
Yinsheng Wang - University of California System
Resource Type: Journal article
Publication Details: Chemical research in toxicology
DOI: 10.1021/acs.chemrestox.6c00094
PMID: 41958033
NLM abbreviation: Chem Res Toxicol
ISSN: 1520-5010
eISSN: 1520-5010
Publisher: American Chemical Society
Grant note: National Institute of Environmental Health Sciences: R21 ES035983
This work was supported by the National Institute of Environmental Health Sciences (R21 ES035983 and U01 ES027252) and Z.H. was supported in part by an NRSA T32 training grant (T32 ES018827).
Language: English
Electronic publication date: 04/09/2026
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pulmonary, Critical Care, and Occupational Medicine; Civil and Environmental Engineering; Occupational and Environmental Health; Research Administration; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Internal Medicine
Record Identifier: 9985152241302771

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