Journal article
Quantitative analysis of HIV-1 variants with the K103N resistance mutation after single-dose nevirapine in women with HIV-1 subtypes A, C, and D
Journal of acquired immune deficiency syndromes (1999), Vol.42(5), pp.610-613
2006
DOI: 10.1097/01.qai.0000221686.67810.20
PMID: 16773030
Abstract
Introduction:
We used a sensitive point mutation assay, LigAmp, to detect and quantify K103N-containing variants in African women who received single-dose nevirapine (NVP) to prevent mother-to-child HIV-1 transmission.
Methods:
Plasma for testing was collected 6 to 8 weeks postpartum from 301 women (144 subtype A, 63 subtype C, and 94 subtype D).
Results:
The portion of women with 0.5% or more K103N-containing variants was lowest for subtype A (60/144, 41.7%) and highest for subtype C (44/63, 69.8%; P < 0.0001). K103N was rarely detected in pre-NVP samples. In a multivariate model, K103N detection was associated with HIV-1 subtype (C > A), after adjusting for log10 delivery viral load, the number of days between NVP dosing and sample collection, age, and parity. Among women with K103N detected: (1) the median %K103N was lower for subtype A (2.2%) than C (11.7%, P = 0.0001) or D (5.5%, P = 0.04), and (2) in a multivariate linear model, higher log10 (%K103N) was associated with HIV subtype (C > A, P = 0.0001; D > A, P = 0.01; and C vs D, no difference), but not other factors.
Conclusions:
After administration of single-dose NVP, K103N was detected more frequently and at higher levels in women with subtypes C and D than A. Further studies are needed to evaluate the clinical significance of NVP-resistant variants in this setting.
Details
- Title: Subtitle
- Quantitative analysis of HIV-1 variants with the K103N resistance mutation after single-dose nevirapine in women with HIV-1 subtypes A, C, and D
- Creators
- Tamara S FLYS - Department of Pathology, The Johns Hopkins School of Medicine, United StatesShu Chen - Department of Epidemiology, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, United StatesNewton KUMWENDA - Department of Epidemiology, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, United StatesTaha E TAHA - Department of Epidemiology, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, United StatesJ. Brooks JACKSON - Department of Pathology, The Johns Hopkins School of Medicine, United StatesSusan H ESHLEMAN - Department of Pathology, The Johns Hopkins School of Medicine, United StatesDana C JONES - Department of Pathology, The Johns Hopkins School of Medicine, United StatesDonald R HOOVER - Department of Statistics and Institute for Health, Health Care Policy and Aging Research, Rutgers University, Piscataway, NJ, United StatesJessica D CHURCH - Department of Pathology, The Johns Hopkins School of Medicine, United StatesSusan A FISCUS - Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesAnthony MWATHA - Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, WA, United StatesLaura A GUAY - Department of Pathology, The Johns Hopkins School of Medicine, United StatesFrancis MMIRO - Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, WA, United StatesPhilippa MUSOKE - Department of Paediatrics, Makerere University, Kampala, Uganda
- Resource Type
- Journal article
- Publication Details
- Journal of acquired immune deficiency syndromes (1999), Vol.42(5), pp.610-613
- Publisher
- Lippincott Williams & Wilkins; Hagerstown, MD
- DOI
- 10.1097/01.qai.0000221686.67810.20
- PMID
- 16773030
- ISSN
- 1525-4135
- eISSN
- 1944-7884
- Language
- English
- Date published
- 2006
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047658602771
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