Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy

Yuan Zou; Steven R Armstrong; Julie L P Jessop

doi:10.1002/jbm.a.32692

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Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy

Journal article

Peer reviewed

Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy

Yuan Zou, Steven R Armstrong and Julie L P Jessop

Journal of biomedical materials research. Part A, Vol.94(1), pp.288-297

07/2010

DOI: 10.1002/jbm.a.32692

PMCID: PMC2875550

PMID: 20186729

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Abstract

The objective was to determine absolute molar concentration of adhesive resin components in the hybrid layer by establishing methods based on Raman spectroscopy fundamentals. The hybrid layer was treated as a three-component system consisting of collagen and an adhesive resin containing two monomers. Adhesive standard specimens and Raman peak area ratios obtained with a 785 nm excitation wavelength were used to construct separate calibration curves for comonomer relative molar concentration and Bis-GMA absolute molar concentration. As collagen and water had no measurable peaks in the fingerprint region, a dilution coefficient K(j) was defined to describe their impact on Raman peak area and to calculate HEMA absolute molar concentration. Methodology was validated using an analogous system containing acetone/ethanol/water. The absolute molar concentration of Bis-GMA and HEMA decreased 87% and 83%, respectively, from the top quarter to the middle of the hybrid layer. Additionally, less Bis-GMA penetrated the hybrid layer than HEMA, as indicated by the approximately 20% decrease in comonomer molar concentration ratio between the adhesive resin layer and the top half of the hybrid layer. Lack of complete monomer infiltration will further challenge dentin-adhesive bond longevity. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

Dentin - chemistry

Methacrylates - analysis

Dentin - cytology

Bisphenol A-Glycidyl Methacrylate - analysis

Biocompatible Materials - chemistry

Dentin-Bonding Agents - analysis

Materials Testing

Spectrum Analysis, Raman - methods

Resin Cements - analysis

Details

Title: Subtitle: Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy
Creators: Yuan Zou - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, USA
Steven R Armstrong
Julie L P Jessop
Resource Type: Journal article
Publication Details: Journal of biomedical materials research. Part A, Vol.94(1), pp.288-297
DOI: 10.1002/jbm.a.32692
PMID: 20186729
PMCID: PMC2875550
NLM abbreviation: J Biomed Mater Res A
ISSN: 1549-3296
eISSN: 1552-4965
Publisher: United States
Grant note: R03 DE 14799 / NIDCR NIH HHS R03 DE014799-02 / NIDCR NIH HHS
Language: English
Date published: 07/2010
Academic Unit: Operative Dentistry; Chemical and Biochemical Engineering
Record Identifier: 9984065714502771

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