Journal article
Quantitative analysis of the regulation of leukocyte chemosensory migration by a vascular prosthetic biomaterial
Journal of materials science. Materials in medicine, Vol.11(6), pp.337-344
06/2000
DOI: 10.1023/A:1008925722623
PMID: 15348014
Abstract
The need for improved, infection-resistant vascular biomaterials calls for more objective evaluation of the immune pathophysiology of implantable prosthetic materials. In this study we have developed a new strategy to quantitatively characterize population-averaged responses of immune cell migration on vascular prosthetic materials. This approach, incorporating a chemokinetically regulated "biomaterial-gel" sandwich configuration, was applied to quantify both random and directed modes of the chemosensory migration of human neutrophil leukocytes on expanded polytetrafluoroethylene (ePTFE). Our studies show that (a) microporous, synthetic materials like ePTFE suppress the basal rate of random cell migration relative to that reported on non-porous control surfaces; (b) stimulation with chemoattractant (formyl peptide) can significantly elevate rates of random and directed migration on ePTFE; and (c) protein conditioning of ePTFE with albumin or immunoglobulin G can differentially modulate the rates and relative proportion of random and directional components of leukocyte migration response to chemoattractant. This, to our knowledge, is the first objective quantitation of chemokinetically regulated cell migration on implantable prosthetic materials.
Details
- Title: Subtitle
- Quantitative analysis of the regulation of leukocyte chemosensory migration by a vascular prosthetic biomaterial
- Creators
- C C Chang - Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ 08854-8058, USAS M LiebermanP V Moghe
- Resource Type
- Journal article
- Publication Details
- Journal of materials science. Materials in medicine, Vol.11(6), pp.337-344
- DOI
- 10.1023/A:1008925722623
- PMID
- 15348014
- ISSN
- 0957-4530
- eISSN
- 1573-4838
- Language
- English
- Date published
- 06/2000
- Academic Unit
- Stead Family Department of Pediatrics; Rheumatology, Allergy, and Immunology
- Record Identifier
- 9984093502902771
Metrics
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