Journal article
Quantitative appraisal of H-2 products in T cell-mediated lysis by allogeneic and syngeneic effector cells
The Journal of immunology (1950), Vol.127(2), pp.500-504
08/1981
DOI: 10.4049/jimmunol.127.2.500
PMID: 6972966
Abstract
In this report we attempted to distinguish between the amount of the serologically defined H-2 products on target cells necessary for their lysis by either alloimmune cytotoxic T lymphocytes (CTL) or by syngeneic, major histocompatibility complex- (MHC) restricted, CTL. Target cells, which were treated with papain and subsequently modified with TNBS, were examined for their susceptibility to lysis by allogeneic and syngeneic CTL. Lysis of papain-treated cells by both types of CTL decreased in a parallel fashion. The decline in lysis correlated with the loss of serologically defined H-2 antigens from the cell surface. Additionally, a panel of cell lines displaying different amounts of H-2 products were examined for their susceptibility to lysis by both sets of effector cells. As with the papain-treated cells, these H-2 'variants' showed a direct relationship between the amount of H-2 products displayed on their surface and their susceptibility to lysis by both the allogeneic and the syngeneic, TNP-specific, effector cells. Whereas papain treatment caused a decrease in the amount of TNP that could be coupled to the target cells, the 'variant' cell lines displayed comparable amounts of TNP after incubation with TNBS. Moreover, further analysis of the papain-treated target cells revealed that it was the amount of the H-2 products, rather than the amount of TNP, which was the limiting variable for recognition by syngeneic CTL. These findings collectively suggest that regardless of whether MHC products act as the restricting element in lytic interactions (as with MHC-restricted TNP-specific syngeneic CTL) or as the substrate for lytic attack (as with alloimmune allogeneic CTL) the quantitative requirements for MHC products are similar.
Details
- Title: Subtitle
- Quantitative appraisal of H-2 products in T cell-mediated lysis by allogeneic and syngeneic effector cells
- Creators
- Rudolf C KuppersZuhair K BallasWilliam R GreenChristopher S Henney
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.127(2), pp.500-504
- DOI
- 10.4049/jimmunol.127.2.500
- PMID
- 6972966
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AI 15383 / NIAID NIH HHS CA 27552 / NCI NIH HHS
- Language
- English
- Date published
- 08/1981
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094612902771
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