Journal article
Quantitative determination of dopamine in human plasma by a highly sensitive LC-MS/MS assay: Application in preterm neonates
Journal of pharmaceutical and biomedical analysis, Vol.117, pp.227-231
01/05/2016
DOI: 10.1016/j.jpba.2015.09.003
PMID: 26372947
Abstract
The determination of dopamine facilitates better understanding of the complex brain disorders in the central nervous system and the regulation of endocrine system, cardiovascular functions and renal functions in the periphery. The purpose of this study was to develop a highly sensitive and reliable assay for the quantification of dopamine in human neonate plasma. Dopamine was extracted from human plasma by strong cation exchange (SCX) solid phase extraction (SPE), and subsequently derivatized with propionic anhydride. The derivatized analyte was separated by a Waters Acquity UPLC BEH C-18 column using gradient elution at 0.4 ml/min with mobile phases A (0.2% formic acid in water [v/v]) and B (MeOH-ACN [v/v, 30:70]). Analysis was performed under positive electrospray ionization tandem mass spectrometer (ESI-MS/MS) in the multiple reaction monitoring (MRM) mode. The stable and relatively non-polar nature of the derivatized analyte enables reliable quantification of dopamine in the range of 10-1000 pg/ml using 200 mu l of plasma sample. The method was validated with intra-day and inter-day precision less than 7%, and the intra-day and inter-day accuracy of 91.9-101.9% and 92.3-102.6%, respectively. The validated assay was applied to quantify dopamine levels in two preterm neonate plasma samples. In conclusion, a sensitive and selective LC-MS/MS method has been developed and validated, and successfully used for the determination of plasma dopamine levels in preterm neonates. (C) 2015 Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Quantitative determination of dopamine in human plasma by a highly sensitive LC-MS/MS assay: Application in preterm neonates
- Creators
- Daping Zhang - University of HoustonLei Wu - University of HoustonDiana S-L. Chow - University of HoustonVincent H. Tam - University of HoustonDanielle R. Rios - Baylor College of Medicine
- Resource Type
- Journal article
- Publication Details
- Journal of pharmaceutical and biomedical analysis, Vol.117, pp.227-231
- Publisher
- Elsevier
- DOI
- 10.1016/j.jpba.2015.09.003
- PMID
- 26372947
- ISSN
- 0731-7085
- eISSN
- 1873-264X
- Number of pages
- 5
- Language
- English
- Date published
- 01/05/2016
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology
- Record Identifier
- 9984353927902771
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