Quantitative estimate of synaptic inputs to striatal neurons during up and down states in vitro

Kim T Blackwell; Uwe Czubayko; Dietmar Plenz

doi:10.1523/jneurosci.23-27-09123.2003

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Quantitative estimate of synaptic inputs to striatal neurons during up and down states in vitro

Journal article

Open access

Peer reviewed

Quantitative estimate of synaptic inputs to striatal neurons during up and down states in vitro

Kim T Blackwell, Uwe Czubayko and Dietmar Plenz

The Journal of neuroscience, Vol.23(27), pp.9123-9132

10/08/2003

DOI: 10.1523/jneurosci.23-27-09123.2003

PMCID: PMC6740825

PMID: 14534246

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Abstract

Up states are prolonged membrane potential depolarizations critical for synaptic integration and action potential generation in cortical and striatal neurons. They commonly result from numerous concurrent synaptic inputs, whereas neurons reside in a down state when synaptic inputs are few. By quantifying the composition, frequency, and amplitude of synaptic inputs for both states, we provide important constraints for state transitions in striatal network dynamics. Up and down states occur naturally in cortex-striatum-substantia nigra cocultures, which were used as an in vitro model in the present study. Spontaneous synaptic inputs during down states were extracted automatically in spiny projection neurons and fast spiking interneurons of the striatum using a newly developed computer algorithm. Consistent with a heterogeneous population of synaptic inputs, PSPs and PSCs showed no correlation in amplitude and rise time and occurred at relatively low frequencies of 10-40 Hz during the down state. The number of synaptic inputs during up states, estimated from the up-state charge and the unitary charge of down-state PSCs, was 217 +/- 44. Given the average up-state duration of 284 +/- 34 msec, synaptic input frequency was approximately 800 Hz during up-states for both neuronal types. Many down-state events reversed at the chloride reversal potential and were blocked by GABA(A) antagonists. The high correlation between up- and down-state reversal potential suggests that despite these drastic changes in synaptic input frequency, the ratio of inhibitory to excitatory currents is similar during both states.

Action Potentials - physiology

Animals

Cerebral Cortex - cytology

Cerebral Cortex - drug effects

Cerebral Cortex - physiology

Coculture Techniques

Corpus Striatum - cytology

Corpus Striatum - drug effects

Corpus Striatum - physiology

GABA Antagonists - pharmacology

GABA-A Receptor Antagonists

gamma-Aminobutyric Acid - metabolism

Interneurons - classification

Interneurons - drug effects

Interneurons - physiology

Membrane Potentials - physiology

Neural Pathways - physiology

Neurons - classification

Neurons - drug effects

Neurons - physiology

Patch-Clamp Techniques

Rats

Rats, Sprague-Dawley

Signal Processing, Computer-Assisted

Statistics as Topic

Substantia Nigra - cytology

Substantia Nigra - drug effects

Substantia Nigra - physiology

Synapses - drug effects

Synapses - physiology

Synaptic Transmission - physiology

Details

Title: Subtitle: Quantitative estimate of synaptic inputs to striatal neurons during up and down states in vitro
Creators: Kim T Blackwell - National Institute of Mental Health
Uwe Czubayko - National Institute of Mental Health
Dietmar Plenz - National Institute of Mental Health
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.23(27), pp.9123-9132
DOI: 10.1523/jneurosci.23-27-09123.2003
PMID: 14534246
PMCID: PMC6740825
NLM abbreviation: J Neurosci
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: K21 MH001141 / NIMH NIH HHS K21-MH01141 / NIMH NIH HHS
Language: English
Date published: 10/08/2003
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute
Record Identifier: 9984446515302771

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