Quantitative microcomputed tomography assessment of intratrabecular, intertrabecular, and cortical bone architecture in a rat model of severe renal osteodystrophy

Tim A J Hopper; Felix W Wehrli; Punam K Saha; Jalal B Andre; Alexander C Wright; Cheryl P Sanchez; Mary B Leonard

doi:10.1097/01.rct.0000238007.19258.3d

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Quantitative microcomputed tomography assessment of intratrabecular, intertrabecular, and cortical bone architecture in a rat model of severe renal osteodystrophy

Journal article

Peer reviewed

Quantitative microcomputed tomography assessment of intratrabecular, intertrabecular, and cortical bone architecture in a rat model of severe renal osteodystrophy

Tim A J Hopper, Felix W Wehrli, Punam K Saha, Jalal B Andre, Alexander C Wright, Cheryl P Sanchez and Mary B Leonard

Journal of computer assisted tomography, Vol.31(2), pp.320-328

03/2007

DOI: 10.1097/01.rct.0000238007.19258.3d

PMID: 17414773

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Abstract

To determine the effects of renal osteodystrophy (ROD) on bone microarchitecture in growing rats. A total of 24 rats underwent 5/6 nephrectomy (NX) and were fed a high-phosphorus diet to induce ROD; another 6 underwent sham NX. In vitro microcomputed tomography images (GEMS, London, Ontario, Canada) were obtained in the femoral metaphysis and midshaft. Trabecular and cortical bone volume/total volume (BV/TV) were significantly lower in NX specimens because of pores within the trabeculae and along the endosteal surface. Topological analysis using component labeling in 3-dimensions verified that trabecular pores connected to the marrow space. After the trabecular pores were filled using a morphological filter, trabecular thickness was significantly increased in NX. In contrast, cortical thickness was significantly decreased in NX compared with controls; however, after filling the endocortical pores, thickness did not differ. The ROD resulted in decreased cortical and trabecular BV/TV, increased porosity, and increased trabecular thickness. Advanced image processing algorithms demonstrated the effects of cortical and trabecular porosity on BV/TV and structure in ROD.

Chronic Kidney Disease-Mineral and Bone Disorder - diagnostic imaging

Severity of Illness Index

Imaging, Three-Dimensional - methods

Rats

Tomography, X-Ray Computed - methods

Male

Bone Density Conservation Agents - administration & dosage

Femur - diagnostic imaging

Chronic Kidney Disease-Mineral and Bone Disorder - diagnosis

Chronic Kidney Disease-Mineral and Bone Disorder - drug therapy

Rats, Sprague-Dawley

Body Weights and Measures - methods

Calcitriol - administration & dosage

Animals

Growth Hormone - administration & dosage

Disease Models, Animal

Details

Title: Subtitle: Quantitative microcomputed tomography assessment of intratrabecular, intertrabecular, and cortical bone architecture in a rat model of severe renal osteodystrophy
Creators: Tim A J Hopper - School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, Australia
Felix W Wehrli
Punam K Saha
Jalal B Andre
Alexander C Wright
Cheryl P Sanchez
Mary B Leonard
Resource Type: Journal article
Publication Details: Journal of computer assisted tomography, Vol.31(2), pp.320-328
Publisher: United States
DOI: 10.1097/01.rct.0000238007.19258.3d
PMID: 17414773
ISSN: 0363-8715
eISSN: 1532-3145
Grant note: K08-DK02503 / NIDDK NIH HHS
Language: English
Date published: 03/2007
Academic Unit: Radiology; Electrical and Computer Engineering
Record Identifier: 9984051732102771

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