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Quantitative model for gene regulation by lambda phage repressor
Journal article   Open access   Peer reviewed

Quantitative model for gene regulation by lambda phage repressor

Gary K Ackers, Alexander D Johnson and Madeline A Shea
Proceedings of the National Academy of Sciences - PNAS, Vol.79(4), pp.1129-1133
02/1982
DOI: 10.1073/pnas.79.4.1129
PMID: 6461856
url
https://doi.org/10.1073/pnas.79.4.1129View
Published (Version of record) Open Access

Abstract

A statistical thermodynamic model has been developed to account for the cooperative interactions of the bacteriophage lambda repressor with the lambda right operator. The model incorporates a general theory for quantitatively interpreting cooperative site-specific equilibrium binding data. Values for all interaction parameters of the model have been evaluated at 37 degrees C, 0.2 M KCl, from results of DNase protection experiments in vitro [A. D. Johnson, B. J. Meyer, & M. Ptashne, Proc. Natl. Acad. Sci. USA (1979) 76, 5061-5065]. With these values, the model predicts repression curves at the divergent promoters PR and PRM that control transcription of genes coding for the regulatory proteins cro and repressor, respectively. At physiological repressor concentrations, repression at PR is predicted to be nearly complete whereas PRM is predicted to remain highly active. The results demonstrate the importance of cooperative interactions between repressor dimers bound to the adjacent operator sites OR1 and OR2 in maintaining a stable lysogenic state and in allowing efficient switchover to the lytic state during induction.
 Mathematics Transcription Factors - physiology Operon Gene Expression Regulation Lysogeny Viral Proteins Viral Regulatory and Accessory Proteins Repressor Proteins - physiology Genes, Viral Models, Genetic Bacteriophage lambda - genetics Virus Activation DNA-Binding Proteins

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