Quercetin inhibits Ca2+ uptake but not Ca2+ release by sarcoplasmic reticulum in skinned muscle fibers

V Shoshan; K P Campbell; D H MacLennan; W Frodis; B A Britt

doi:10.1073/pnas.77.8.4435

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Quercetin inhibits Ca2+ uptake but not Ca2+ release by sarcoplasmic reticulum in skinned muscle fibers

Journal article

Open access

Peer reviewed

Quercetin inhibits Ca2+ uptake but not Ca2+ release by sarcoplasmic reticulum in skinned muscle fibers

V Shoshan, K P Campbell, D H MacLennan, W Frodis and B A Britt

Proceedings of the National Academy of Sciences - PNAS, Vol.77(8), pp.4435-4438

08/1980

DOI: 10.1073/pnas.77.8.4435

PMCID: PMC349858

PMID: 6449007

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Abstract

Quercetin inhibited Ca2+-dependent ATP hydrolysis, ATP-dependent Ca2+ uptake, chelator-induced [ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] Ca2+ release, and ATP synthesis coupled to Ca2+ release in isolated vesicles of sarcoplasmic reticulum. Use of this inhibitor permitted evaluation of whether Ca2+ release from sarcoplasmic reticulum in situ occurs through a reversal of the uptake pathway. Release of Ca2+ from the sarcoplasmic reticulum of skinned muscle fibers can be detected by the measurement of tension in the fiber. If the sarcoplasmic reticulum of these preparations is first allowed to accumulate Ca2+, tension development may be induced by the addition of Ca2+ itself or of caffeine to the bathing medium or by depolarization with Cl-. The presence of quercetin during the loading phase inhibited Ca2+ uptake by sarcoplasmic reticulum in situ. When quercetin was added together with initiators of tension development, however, the rate of tension development was enhanced 4- to 7-fold and the relaxation rate of the fibers was greatly inhibited. These results suggest that quercetin had no effect on Ca2+ release in skinned fiber; its effect on Ca2+ reuptake could account for the apparent enhancement of the release rate and for the prolonged relaxation time. These observations rule out reversal of the Ca2+ pump as the mechanism of Ca2+ release in situ.

Calcium-Transporting ATPases - antagonists & inhibitors

Rabbits

Animals

Quercetin - pharmacology

Calcium - metabolism

Chlorides - pharmacology

Muscle Contraction - drug effects

Sarcoplasmic Reticulum - drug effects

Humans

Caffeine - pharmacology

Flavonoids - pharmacology

Sarcoplasmic Reticulum - metabolism

Details

Title: Subtitle: Quercetin inhibits Ca2+ uptake but not Ca2+ release by sarcoplasmic reticulum in skinned muscle fibers
Creators: V Shoshan
K P Campbell - University of Iowa, Molecular Physiology and Biophysics
D H MacLennan
W Frodis
B A Britt
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.77(8), pp.4435-4438
DOI: 10.1073/pnas.77.8.4435
PMID: 6449007
PMCID: PMC349858
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; United States
Language: English
Date published: 08/1980
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984025080302771

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