Quinocetone triggers oxidative stress and induces cytotoxicity and genotoxicity in human peripheral lymphocytes of both genders

Wei Yang; Juan Fu; Xiao Xiao; Hong Yan; Wei Bao; Di Wang; Liping Hao; Andreas K Nussler; Ping Yao; Liegang Liu

doi:10.1002/jsfa.5891

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Quinocetone triggers oxidative stress and induces cytotoxicity and genotoxicity in human peripheral lymphocytes of both genders

Journal article

Peer reviewed

Quinocetone triggers oxidative stress and induces cytotoxicity and genotoxicity in human peripheral lymphocytes of both genders

Wei Yang, Juan Fu, Xiao Xiao, Hong Yan, Wei Bao, Di Wang, Liping Hao, Andreas K Nussler, Ping Yao and Liegang Liu

Journal of the science of food and agriculture, Vol.93(6), pp.1317-1325

04/2013

DOI: 10.1002/jsfa.5891

PMID: 23027643

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Abstract

Quinocetone has been widely used as an animal growth promoter in China. However, available data showed that QCT has potential genotoxicity. This study was conducted to investigate the cytotoxicity and genotoxicity of QCT in human lymphocytes. CCK-8 assay demonstrated the severe inhibitory effects by QCT in a dose- and time-dependent manner. DNA damage analysis using alkalic Comet assay revealed a pronounced increase of DNA fragmentation in cells. In contrast, DNA damage was significantly decreased after incubation with S9 mix. This finding demonstrated that the intermediate metabolites of this drug exerted lower genotoxicity than its parent drugs. We further described chromosomal damage induced by this drug employing cytokinesis-block micronucleus assay. The micronucleus frequency was significantly increased in quinocetone groups as compared to controls. Similar to the observation in Comet assay, incorporation of S9 mix in the cytokinesis-block micronucleus assay could markedly alleviate the chromosomal damage. Moreover, QCT could invoke increase of reactive oxygen species generation in cells. Intriguingly, the toxicity of QCT was more prominent in samples from males than those from females under the same conditions. QCT could induce potential cytotoxicity and genotoxicity in human lymphocytes.

Oxidative Stress

China

Quinoxalines - adverse effects

Reactive Oxygen Species - metabolism

Food Contamination

Humans

Male

Dose-Response Relationship, Drug

Micronucleus Tests

Young Adult

Cattle

Mutagenicity Tests

Adult

Female

Cell Culture Techniques

Lymphocytes - metabolism

Micronuclei, Chromosome-Defective

Chromosomes, Human - drug effects

Cytotoxins - adverse effects

Comet Assay

Animals

Mutagens - adverse effects

Lymphocytes - drug effects

Sex Factors

DNA Damage

Details

Title: Subtitle: Quinocetone triggers oxidative stress and induces cytotoxicity and genotoxicity in human peripheral lymphocytes of both genders
Creators: Wei Yang - Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Juan Fu
Xiao Xiao - Huazhong University of Science and Technology
Hong Yan
Wei Bao
Di Wang
Liping Hao
Andreas K Nussler
Ping Yao
Liegang Liu
Resource Type: Journal article
Publication Details: Journal of the science of food and agriculture, Vol.93(6), pp.1317-1325
Publisher: England
DOI: 10.1002/jsfa.5891
PMID: 23027643
ISSN: 0022-5142
eISSN: 1097-0010
Language: English
Date published: 04/2013
Academic Unit: Epidemiology; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9983995047502771

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