Journal article
RAD18-dependent Recruitment of SNM1A to DNA Repair Complexes by a Ubiquitin-binding Zinc Finger
The Journal of biological chemistry, Vol.285(25), pp.19085-19091
06/18/2010
DOI: 10.1074/jbc.M109.100032
PMCID: PMC2885187
PMID: 20385554
Abstract
SNM1A is a member of the SNM1 family of nucleases required for cellular processing of interstrand DNA crosslinks (ICLs). Little is known about the molecular function of SNM1A, in terms of its recruitment to ICL lesions or its DNA damage processing activity. Here we show that SNM1A contains a functional PIP box (PCNA-interacting protein box) and a UBZ (ubiquitin binding zinc finger), required for assembly of SNM1A into nuclear focus. Moreover, RAD18-dependent monoubiquitination of PCNA is required for Mitomycin C and Ultraviolet Light inducible SNM1A nuclear focus assembly. Taken together, our results identify a novel RAD18-PCNA(Ub)-SNM1A pathway required for nuclear focus formation and ICL resistance.
Details
- Title: Subtitle
- RAD18-dependent Recruitment of SNM1A to DNA Repair Complexes by a Ubiquitin-binding Zinc Finger
- Creators
- Kailin Yang - Dana-Farber Cancer InstituteGeorge-Lucian Moldovan - From the Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115Alan D. D'Andrea - From the Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.285(25), pp.19085-19091
- Publisher
- Elsevier Inc
- DOI
- 10.1074/jbc.M109.100032
- PMID
- 20385554
- PMCID
- PMC2885187
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 06/18/2010
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984696580402771
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