Journal article
RAD51-Dependent Break-Induced Replication Differs in Kinetics and Checkpoint Responses from RAD51-Mediated Gene Conversion
Molecular and cellular biology, Vol.25(3), pp.933-944
02/2005
DOI: 10.1128/MCB.25.3.933-944.2005
PMCID: PMC544012
PMID: 15657422
Abstract
Diploid Saccharomyces cells experiencing a double-strand break (DSB) on one homologous chromosome repair the break by RAD51-mediated gene conversion >98% of the time. However, when extensive homologous sequences are restricted to one side of the DSB, repair can occur by both RAD51-dependent and RAD51-independent break-induced replication (BIR) mechanisms. Here we characterize the kinetics and checkpoint dependence of RAD51-dependent BIR when the DSB is created within a chromosome. Gene conversion products appear within 2 h, and there is little, if any, induction of the DNA damage checkpoint; however, RAD51-dependent BIR occurs with a further delay of 2 to 4 h and cells arrest in response to the G2/M DNA damage checkpoint. RAD51-dependent BIR does not require special facilitating sequences that are required for a less efficient RAD51-independent process. RAD51-dependent BIR occurs efficiently in G2-arrested cells. Once repair is initiated, the rate of repair replication during BIR is comparable to that of normal DNA replication, as copying of >100 kb is completed less than 30 min after repair DNA synthesis is detected close to the DSB.
Details
- Title: Subtitle
- RAD51-Dependent Break-Induced Replication Differs in Kinetics and Checkpoint Responses from RAD51-Mediated Gene Conversion
- Creators
- Anna Malkova - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MassachusettsMaria L Naylor - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MassachusettsMiyuki Yamaguchi - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MassachusettsGrzegorz Ira - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MassachusettsJames E Haber - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.25(3), pp.933-944
- DOI
- 10.1128/MCB.25.3.933-944.2005
- PMID
- 15657422
- PMCID
- PMC544012
- NLM abbreviation
- Mol Cell Biol
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 02/2005
- Academic Unit
- Biology
- Record Identifier
- 9984217420602771
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