Journal article
RAD51 discrimination between single- and double-strand DNA: a matter of flexibility and enthalpy
The EMBO journal, Vol.39(7), pp.e104547-n/a
04/01/2020
DOI: 10.15252/embj.2020104547
PMCID: PMC7110141
PMID: 32090346
Abstract
In selecting ssDNA over dsDNA, the RAD51 DNA strand exchange protein has to overcome the entropy associated with straightening of single-strand DNA upon nucleoprotein filament formation. New work in The EMBO Journal (Paoletti et al, 2020), combined biophysical analysis of the RAD51-ssDNA interaction with mathematical modeling to show that the flexibility of DNA positively correlates with nucleation and extension of the RAD51 nucleoprotein filament and that the entropic penalty associated with restricting ssDNA flexibility is offset by a strong RAD51-RAD51 interaction within the nucleoprotein filament.
Details
- Title: Subtitle
- RAD51 discrimination between single- and double-strand DNA: a matter of flexibility and enthalpy
- Creators
- Shyamal Subramanyam - Memorial Sloan Kettering Cancer CenterMaria Spies - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.39(7), pp.e104547-n/a
- DOI
- 10.15252/embj.2020104547
- PMID
- 32090346
- PMCID
- PMC7110141
- NLM abbreviation
- EMBO J
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Publisher
- Wiley
- Number of pages
- 3
- Language
- English
- Date published
- 04/01/2020
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984293087002771
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