Journal article
RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site
Genes & development, Vol.15(9), pp.1055-1060
05/01/2001
DOI: 10.1101/gad.875901
PMCID: PMC312680
PMID: 11331601
Abstract
Without the
RAD51
strand exchange protein,
Saccharomyces cerevisiae
cannot repair a double-strand break (DSB) by gene conversion. However, cells can repair DSBs by recombination-dependent, break-induced replication (BIR).
RAD51
-independent BIR is initiated more than 13 kb from the DSB. Repair depends on a 200-bp sequence adjacent to
ARS310
, located ∼34 kb centromere-proximal to the DSB, but does not depend on the origin activity of
ARS310
. We conclude that the ability of a recombination-induced replication fork to copy >130 kb to the end of the chromosome depends on a special site that enhances assembly of a processive repair replication fork.
Details
- Title: Subtitle
- RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site
- Creators
- Anna Malkova - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USALaurence Signon - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USAChristopher B Schaefer - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USAMaria L Naylor - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USAJames F Theis - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USACarol S Newlon - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USAJames E Haber - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA; Department of Microbiology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School and University of Medicine and Dentistry of New Jersey-Graduate School of Biomedical Sciences, Newark, New Jersey 07103, USA
- Resource Type
- Journal article
- Publication Details
- Genes & development, Vol.15(9), pp.1055-1060
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/gad.875901
- PMID
- 11331601
- PMCID
- PMC312680
- ISSN
- 0890-9369
- eISSN
- 1549-5477
- Language
- English
- Date published
- 05/01/2001
- Academic Unit
- Biology
- Record Identifier
- 9984217530802771
Metrics
7 Record Views