RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK)

Ye Zhang; Qiwei Zhai; Yi Luo; Martin E Dorf

doi:10.1074/jbc.M112442200

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RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK)

Journal article

Open access

Peer reviewed

RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK)

Ye Zhang, Qiwei Zhai, Yi Luo and Martin E Dorf

The Journal of biological chemistry, Vol.277(21), pp.19042-19048

05/24/2002

DOI: 10.1074/jbc.M112442200

PMID: 11893739

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Abstract

RANTES (regulated on activation normal T cell expressed and secreted) (> or =10 ng/ml) stimulates the induction of KC and other chemokines in astrocytes. Elements of the signal transduction pathway controlling this response were identified. RANTES induced phosphorylation of MEK, ERK1/2, p90 ribosomal S6 kinases (RSK), and cAMP-response element-binding protein (CREB) in astrocytes. U0126, a pharmacological inhibitor of MEK, blocked the phosphorylation of the downstream elements ERK, RSK, and CREB, inhibited chemokine synthesis, and reduced transcription from a KC promoter construct. Dominant negative mutants of RSK or CREB blocked the transcription driven by the KC promoter. Finally, RANTES treatment induces nuclear translocation of phosphorylated RSK in astrocytes. This novel role for RSK in signaling chemokine responses and synthesis in astrocytes may contribute to the amplification mechanisms responsible for prolonging inflammatory responses in the central nervous system.

Animals

Astrocytes - enzymology

Astrocytes - metabolism

Chemokine CCL5 - physiology

Chemokines - genetics

Enzyme Activation

Enzyme-Linked Immunosorbent Assay

MAP Kinase Signaling System

Mice

Mice, Inbred BALB C

Phosphorylation

Protein Transport

Recombinant Proteins - metabolism

Ribosomal Protein S6 Kinases - metabolism

Transcription, Genetic - physiology

Tumor Necrosis Factor-alpha - biosynthesis

Details

Title: Subtitle: RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK)
Creators: Ye Zhang - Harvard University
Qiwei Zhai - Harvard University
Yi Luo - Harvard University
Martin E Dorf - Harvard University
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.277(21), pp.19042-19048
DOI: 10.1074/jbc.M112442200
PMID: 11893739
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Grant note: NS37284 / NINDS NIH HHS CA67416 / NCI NIH HHS
Language: English
Date published: 05/24/2002
Academic Unit: Urology
Record Identifier: 9984320067902771

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