RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model

Wujiang Liu; Barry R. DeYoung; Xiaohong Chen; David P. Evanoff; Yi Luo

doi:10.1016/j.jaut.2007.10.005

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RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model

Journal article

Open access

Peer reviewed

RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model

Wujiang Liu, Barry R. DeYoung, Xiaohong Chen, David P. Evanoff and Yi Luo

Journal of autoimmunity, Vol.30(4), pp.257-265

2008

DOI: 10.1016/j.jaut.2007.10.005

PMCID: PMC2441447

PMID: 18162370

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https://www.ncbi.nlm.nih.gov/pmc/articles/2441447View

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Abstract

Interstitial cystitis (IC) is a chronic inflammatory condition of the urinary bladder with a strong autoimmune component. Currently, the major challenge in IC treatment is the development of effective therapies. RDP58 is a novel d-amino acid decapeptide with potent immunosuppressive activity. In this study, we investigated whether RDP58 was effective as an intravesical agent for treating bladder autoimmune inflammation in a transgenic mouse model (URO–OVA mice). URO-OVA mice were adoptively transferred with syngeneic activated splenocytes of OT-I mice transgenic for the OVA-specific CD8 + TCR for cystitis induction and treated intravesically with RDP58 at days 0 and 3. Compared with controls, the RDP58-treated bladders showed markedly reduced histopathology and expressions of mRNAs and proteins of TNF-α, NGF and substance P. To determine whether the inhibition of bladder inflammation by RDP58 was due to the interference with effector T cells, we treated the cells with RDP58 in vitro. Cells treated with RDP58 showed reduced production of TNF-α and IFN-γ as well as apoptotic death. Collectively, these results indicate that RDP58 is effective for treating T cell-mediated experimental autoimmune cystitis and may serve as a useful intravesical agent for the treatment of autoimmune-associated bladder inflammation such as IC.

Autoimmunity

Cystitis

Inflammation

RDP58

Urinary bladder

Details

Title: Subtitle: RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model
Creators: Wujiang Liu - Roy J. and Lucille A. Carver College of Medicine
Barry R. DeYoung - University of Iowa
Xiaohong Chen - University of Iowa
David P. Evanoff - Roy J. and Lucille A. Carver College of Medicine
Yi Luo - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Journal of autoimmunity, Vol.30(4), pp.257-265
DOI: 10.1016/j.jaut.2007.10.005
PMID: 18162370
PMCID: PMC2441447
NLM abbreviation: J Autoimmun
ISSN: 0896-8411
eISSN: 1095-9157
Publisher: Elsevier Ltd
Language: English
Date published: 2008
Academic Unit: Urology
Record Identifier: 9984320852202771

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