Journal article
REPEATED MEASUREMENT OF MECHANICAL PROPERTIES IN VIABLE OSTEOCHONDRAL EXPLANTS FOLLOWING A SINGLE BLUNT IMPACT INJURY
Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, Vol.225(10), pp.993-1002
10/2011
DOI: 10.1177/0954411911413063
PMCID: PMC3684069
PMID: 22204121
Abstract
The objective of this work was to develop a method for repeated same-site measurement of mechanical properties suitable for the detection of degenerative changes in a biologically active explant model after a single blunt impact injury. Focal blunt impact injuries to articular surfaces lead to local cartilage degeneration and loss of mechanical properties. We employed a repeated measurement methodology to determine variations in mechanical same-site properties before and after injury in living cartilage, with the hypothesis that normalization with initial mechanical properties may provide a clearer evaluation of impact effects and improve our understanding of the biologic responses to impact injury. Bovine osteochondral explants were cultured for up to 14 days after impact injury. Indentation tests were performed before and after impact injury to assess relative changes in mechanical properties. Creep strain increased significantly in impacted explants after 7 days and in both impacted and control explants after 14 days. Further analysis at 14 days revealed decreases in stretch factor beta, creep time constant and local compressive modulus. A repeated measures methodology reliably detected changes in the mechanical behaviour of viable osteochondral explants after a single impact injury.
Details
- Title: Subtitle
- REPEATED MEASUREMENT OF MECHANICAL PROPERTIES IN VIABLE OSTEOCHONDRAL EXPLANTS FOLLOWING A SINGLE BLUNT IMPACT INJURY
- Creators
- Prem S Ramakrishnan - Ignacio Ponseti Cell and Molecular Biology lab, Department of Orthopedics and Rehabilitation, University of Iowa Hospitals, Iowa City, IowaDouglas R Pedersen - Orthopedic Biomechanics Laboratory, Department of Orthopedics and Rehabilitation, University of Iowa Hospitals, Iowa City, IowaNicholas J Stroud - Orthopedic Biomechanics Laboratory, Department of Orthopedics and Rehabilitation, University of Iowa Hospitals, Iowa City, IowaDaniel J McCabe - Ignacio Ponseti Cell and Molecular Biology lab, Department of Orthopedics and Rehabilitation, University of Iowa Hospitals, Iowa City, IowaJames A Martin - Ignacio Ponseti Cell and Molecular Biology lab, Department of Orthopedics and Rehabilitation, University of Iowa Hospitals, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, Vol.225(10), pp.993-1002
- DOI
- 10.1177/0954411911413063
- PMID
- 22204121
- PMCID
- PMC3684069
- NLM abbreviation
- Proc Inst Mech Eng H
- ISSN
- 0954-4119
- eISSN
- 2041-3033
- Grant note
- P50 AR055533 || AR / National Institute of Arthritis and Musculoskeletal and Skin Diseases : NIAMS
- Language
- English
- Date published
- 10/2011
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Emergency Medicine; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
- Record Identifier
- 9984040268602771
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