Journal article
RIG-I Detects mRNA of Intracellular Salmonella enterica Serovar Typhimurium during Bacterial Infection
mBio, Vol.5(2), pp.e01006-e01014
05/2014
DOI: 10.1128/mBio.01006-14
PMCID: PMC3977358
PMID: 24692634
Abstract
ABSTRACT The cytoplasmic helicase RIG-I is an established sensor for viral 5′-triphosphorylated RNA species. Recently, RIG-I was also implicated in the detection of intracellular bacteria. However, little is known about the host cell specificity of this process and the bacterial pathogen-associated molecular pattern (PAMP) that activates RIG-I. Here we show that RNA of Salmonella enterica serovar Typhimurium activates production of beta interferon in a RIG-I-dependent fashion only in nonphagocytic cells. In phagocytic cells, RIG-I is obsolete for detection of Salmonella infection. We further demonstrate that Salmonella mRNA reaches the cytoplasm during infection and is thus accessible for RIG-I. The results from next-generation sequencing analysis of RIG-I-associated RNA suggest that coding bacterial mRNAs represent the activating PAMP. IMPORTANCE S. Typhimurium is a major food-borne pathogen. After fecal-oral transmission, it can infect epithelial cells in the gut as well as immune cells (mainly macrophages, dendritic cells, and M cells). The innate host immune system relies on a growing number of sensors that detect pathogen-associated molecular patterns (PAMPs) to launch a first broad-spectrum response to invading pathogens. Successful detection of a given pathogen depends on colocalization of host sensors and PAMPs as well as potential countermeasures of the pathogen during infection. RIG-I-like helicases were mainly associated with detection of RNA viruses. Our work shows that S. Typhimurium is detected by RIG-I during infection specifically in nonimmune cells.
S. Typhimurium is a major food-borne pathogen. After fecal-oral transmission, it can infect epithelial cells in the gut as well as immune cells (mainly macrophages, dendritic cells, and M cells). The innate host immune system relies on a growing number of sensors that detect pathogen-associated molecular patterns (PAMPs) to launch a first broad-spectrum response to invading pathogens. Successful detection of a given pathogen depends on colocalization of host sensors and PAMPs as well as potential countermeasures of the pathogen during infection. RIG-I-like helicases were mainly associated with detection of RNA viruses. Our work shows that S. Typhimurium is detected by RIG-I during infection specifically in nonimmune cells.
Details
- Title: Subtitle
- RIG-I Detects mRNA of Intracellular Salmonella enterica Serovar Typhimurium during Bacterial Infection
- Creators
- Mirco Schmolke - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USAJenish R Patel - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Graduate School of Biomedical Sciences, Microbiology Training Area, Icahn School of Medicine at Mount Sinai, New York, New York, USAElisa de Castro - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USAMaria T Sánchez-Aparicio - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USAMelissa B Uccellini - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USAJennifer C Miller - Graduate School of Biomedical Sciences, Microbiology Training Area, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USABalaji Manicassamy - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USATakashi Satoh - Immunology Frontier Research Center, Osaka University, Osaka, JapanTaro Kawai - Immunology Frontier Research Center, Osaka University, Osaka, JapanShizuo Akira - Immunology Frontier Research Center, Osaka University, Osaka, JapanMiriam Merad - Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USAAdolfo García-Sastre - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA, Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA
- Contributors
- W. Ian Lipkin (Editor)
- Resource Type
- Journal article
- Publication Details
- mBio, Vol.5(2), pp.e01006-e01014
- DOI
- 10.1128/mBio.01006-14
- PMID
- 24692634
- PMCID
- PMC3977358
- NLM abbreviation
- mBio
- ISSN
- 2161-2129
- eISSN
- 2150-7511
- Language
- English
- Date published
- 05/2014
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083885902771
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