R.I.P. to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance

Elizabeth M Boehm; M Todd Washington

doi:10.1002/bies.201600116

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R.I.P. to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance

Journal article

Peer reviewed

R.I.P. to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance

Elizabeth M Boehm and M Todd Washington

BioEssays, Vol.38(11), pp.1117-1122

11/2016

DOI: 10.1002/bies.201600116

PMCID: PMC5341575

PMID: 27539869

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Abstract

Many proteins responsible for genome maintenance interact with one another via short sequence motifs. The best known of these are PIP motifs, which mediate interactions with the replication protein PCNA. Others include RIR motifs, which bind the translesion synthesis protein Rev1, and MIP motifs, which bind the mismatch repair protein Mlh1. Although these motifs have similar consensus sequences, they have traditionally been viewed as separate motifs, each with their own target protein. In this article, we review several recent studies that challenge this view. Taken together, they imply that these different motifs are not distinct entities. Instead, there is a single, broader class of motifs, which we call "PIP-like" motifs, which have overlapping specificities and are capable of binding multiple target proteins. Given this, we must reassess the role of these motifs in forming the network of interacting proteins responsible for genome maintenance.

DNA Repair

DNA Replication

Saccharomyces cerevisiae - metabolism

Recombination, Genetic

Saccharomyces cerevisiae - genetics

Humans

Saccharomyces cerevisiae Proteins - metabolism

Nuclear Proteins - metabolism

Protein Interaction Domains and Motifs

MutL Protein Homolog 1 - metabolism

Nucleotidyltransferases - metabolism

Proliferating Cell Nuclear Antigen - metabolism

Details

Title: Subtitle: R.I.P. to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance
Creators: Elizabeth M Boehm
M Todd Washington - Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, USA. todd-washington@uiowa.edu
Resource Type: Journal article
Publication Details: BioEssays, Vol.38(11), pp.1117-1122
DOI: 10.1002/bies.201600116
PMID: 27539869
PMCID: PMC5341575
NLM abbreviation: Bioessays
ISSN: 0265-9247
eISSN: 1521-1878
Publisher: United States
Grant note: T32 GM067795 / NIGMS NIH HHS R01 GM108027 / NIGMS NIH HHS R01 GM081433 / NIGMS NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 11/2016
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024510802771

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