Journal article
RNA-Seq Experiment and Data Analysis
Methods in molecular biology (Clifton, N.J.), Vol.1366, pp.99-114
2016
DOI: 10.1007/978-1-4939-3127-9_9
PMID: 26585130
Abstract
With the ability to obtain tens of millions of reads, high-throughput messenger RNA sequencing (RNA-Seq) data offers the possibility of estimating abundance of isoforms and finding novel transcripts. In this chapter, we describe a protocol to construct an RNA-Seq library for sequencing on Illumina NGS platforms, and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of differential gene expression in control versus 17β-estradiol treatment of in vivo or in vitro systems.
Details
- Title: Subtitle
- RNA-Seq Experiment and Data Analysis
- Creators
- Hanquan Liang - McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USAErliang Zeng - Department of Computer Science, University of South Dakota, 414 E. Clark Street, Vermillion, SD, 57069, USA. Erliang.Zeng@usd.edu
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1366, pp.99-114
- Publisher
- United States
- DOI
- 10.1007/978-1-4939-3127-9_9
- PMID
- 26585130
- eISBN
- 149393127X; 9781493931279
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2016
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
- Record Identifier
- 9984070862502771
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