RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation

Chunsheng Dong; Guoping Zhao; Mei Zhong; Yan Yue; Li Wu; Sidong Xiong

doi:10.1016/j.gene.2013.02.015

Back

RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation

Journal article

Peer reviewed

RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation

Chunsheng Dong, Guoping Zhao, Mei Zhong, Yan Yue, Li Wu and Sidong Xiong

Gene, Vol.519(2), pp.279-287

05/01/2013

DOI: 10.1016/j.gene.2013.02.015

PMCID: PMC3666862

PMID: 23458880

View Online

Abstract

Monocytes can be differentiated into macrophages in vivo and these cells play an important role in innate and adaptive immune responses. To reveal the global gene transcription change that occurs during monocyte to macrophage differentiation, we performed genome-wide RNA sequencing and analyses in human primary monocytes and monocyte-derived macrophages. We show that 1208 genes (with >twofold differences) were differentially expressed in macrophages compared with monocytes, including 800 upregulated and 408 downregulated genes. Gene ontology, pathway, and protein–protein interaction analyses indicated that the upregulated genes were related to macrophage functions in phagocytosis, metabolic processes, and cell cycle. The majority of downregulated genes comprised genes involved in the inflammatory response and locomotion. Genes encoding transcription regulatory factors, such as FOXO1, RUNX3, NF-κB1, and C/EBP δ, were highly expressed in monocytes and appeared to function in significant transcriptional repression, resulting in slight metabolic activity. Our transcriptome comparison between human monocytes and monocyte-derived macrophages using RNA sequencing revealed novel molecules and pathways associated with the differentiation process. These molecules and pathways may represent candidate targets involved in the pathophysiology of these important immune cells.

Monocytes

RNA-seq

M-CSF

Monocyte-derived macrophages

Transcriptome

Details

Title: Subtitle: RNA sequencing and transcriptomal analysis of human monocyte to macrophage differentiation
Creators: Chunsheng Dong - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Science, Soochow University, Suzhou 215123, PR China
Guoping Zhao - SUDA-SCBIT Joint Laboratory of Comparative and Immunological Genomics (JL-CIG), Institutes of Biology and Medical Science, Soochow University, Suzhou 215123, PR China
Mei Zhong - Center for Stem Cell Research, Yale University, New Haven, CT 06510, USA
Yan Yue - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Science, Soochow University, Suzhou 215123, PR China
Li Wu - Center for Retrovirus Research, Department of Veterinary Bioscience, The Ohio State University, Columbus, OH 43210, USA
Sidong Xiong - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Science, Soochow University, Suzhou 215123, PR China
Resource Type: Journal article
Publication Details: Gene, Vol.519(2), pp.279-287
DOI: 10.1016/j.gene.2013.02.015
PMID: 23458880
PMCID: PMC3666862
NLM abbreviation: Gene
ISSN: 0378-1119
eISSN: 1879-0038
Publisher: Elsevier B.V
Grant note: name: China NSFC, award: 81101257, 31270977; name: Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT); name: Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD); name: NIH, award: AI098524
Language: English
Date published: 05/01/2013
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001226702771

Metrics

34 Record Views

32 Times Cited - Web of Science

See more details