Logo image
RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia
Journal article   Open access   Peer reviewed

RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia

Hannah Voic, Xiuying Li, Jun-Ho Jang, Chunbin Zou, Prithu Sundd, Jonathan Alder, Mauricio Rojas, Divay Chandra, Scott Randell, Rama K. Mallampalli, …
BMC genomics, Vol.20(1), 22
01/09/2019
DOI: 10.1186/s12864-018-5409-z
PMID: 30626320
url
https://doi.org/10.1186/s12864-018-5409-zView
Published (Version of record) Open Access

Abstract

Abstract Background Aging is affected by genetic and environmental factors, and cigarette smoking is strongly associated with accumulation of senescent cells. In this study, we wanted to identify genes that may potentially be beneficial for cell survival in response to cigarette smoke and thereby may contribute to development of cellular senescence. Results Primary human bronchial epithelial cells from five healthy donors were cultured, treated with or without 1.5% cigarette smoke extract (CSE) for 24 h or were passaged into replicative senescence. Transcriptome changes were monitored using RNA-seq in CSE and non-CSE exposed cells and those passaged into replicative senescence. We found that, among 1534 genes differentially regulated during senescence and 599 after CSE exposure, 243 were altered in both conditions, representing strong enrichment. Pathways and gene sets overrepresented in both conditions belonged to cellular processes that regulate reactive oxygen species, proteasome degradation, and NF-κB signaling. Conclusions Our results offer insights into gene expression responses during cellular aging and cigarette smoke exposure, and identify potential molecular pathways that are altered by cigarette smoke and may also promote airway epithelial cell senescence.

Details

Metrics

Logo image